Questions: 1. How much of the culture media is needed for all female students? 2. How much of the culture media is needed for all male students? 3. How much of the culture media is needed for the whole class? 4. How much of the culture media if each students are required to prepare 3 nutrient broth tubes?
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- A class of 15 students (8 males and 7 females) will need to culture an unknown bacteria for a specific activity where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to prepare. agarplate: 25mL Agar slant: 10mL Broth tube: 8mL Nutrient Agar: Yeast extract: 2g/L peptone: 5g/L Sodium chloride: 5g/L Agar powder: 15g/Lnt ● ● S ● Paragraph F 2. To determine if the type of agar affects bacterial growth, a scientist cultures on fou different types of agar. Five petri dishes are set up to collect results: One with nutrient agar and E. coli One with mannitol-salt agar and E. coli One with MacConkey agar and E. coli One with LB agar and E. coli Styles One with nutrient agar but NO E. coli EdIn disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar plate? in the agar directly below the disc O in the agar halfway between the disc and the petri dish wall O in the agar furthest away from the disc the cehmical is equally distributed throughout the agar
- Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with mold
- The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolismYou are tasked with quantifying bacteria in your soil sample. You resuspend 1.0g of soil in 10 ml 1x PBS, then do a 10-fold serial dilution of your soil sample. You plate 150 µl onto each plate. The 104 dilution plate contains 175 colonies after 2 days of incubation, how many bacteria do you estimate were present in your initial soil sample? (# colonies/volume plated in ul) * dilution factor * ul convert to ml * volume suspended soil (ml/g) = Read the formatting instructions below before entering your answer! 1. Make sure your answer is in correct scientific notation using an "e" to indicate the exponent! For example, 2500 would be 2.5e3, 0.0066 would be 6.6e-3. 2. For answers greater than 1, round up to the nearest tenth. For example, 2515 would be 2.5e3 and 51510 would be 5.2e4.A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 Celcius
- When testing the efficacy of an antibiotic against bacteria on an agar plate, it is important to spread the bacteria evenly across the plate before you add the antibiotic. Why do the bacteria need to be applied evenly? None of the following are true All of the following are true If you add more bacteria in some areas compared to others, it might obscure the effect of the antibiotic If the area right next to the antibiotic initially received relatively few bacterial cells, that might lead you to overestimate the effectiveness of the antibiotic If the area right next to the antibiotic initially received a relatively large number of bacterial cells, that might lead you to underestimate the effectiveness of the antibioticSubculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates. Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each. Description: Isolate A – Describe: Colony morphology: Medium & incubation temperature: Isolate B – Describe: Colony morphology: Medium & incubation temperature:procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slant