Question: Estimate the pl of the tripeptide ? Step 1: Determine what can be protonated/deprotonated Step 2: Estimate where the net charge of the protein would be = 0 Step 3: Use the pka's that bracket the neutral form. +H₂N H H H соон сно CH₂ coo- CH3 CH₂ COO H3N pka= 9.82 pka= 3.86 O O=C H H H H CH3 CH3 COO pka= 2.19

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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Question: Estimate the pl of the tripeptide ?
Step 1: Determine what can be
protonated/deprotonated
Abundance
Step 2: Estimate where the net
charge of the protein would be = 0
Step 3: Use the pka's that bracket
the neutral form.
+H₂N
COOH
تیار کیا ہے
CH₂
H
CC
H
+1
H₂N
H
COOH
CH3
CH3
0 1 2
cook
CH₂
coof
H
pkg= 2.19
O
H
H₂N
No charge
H
H
соон
CH3
CH3
3 4
pka= 3.86
Coo
COO™
CH₂ H
H H
coof
CH3
CH3
pka= 4.25
COO
- 2
5 6
6
Coo
J.
CH₂
7
pH
O
H H
8
H
coo-
CH3
CH3
COO"
9
+
Н.
H3N
pka= 9.82
10
pka= 9.82
pka= 3.86
CH₂
H₂N
11
H H H
coo
CH₂ p
- 3
O=C
H H H
12
H
pka= 4.25
O
CH3
1
CH3
coo-
CH3
CH3
COO
13
COO pka= 2.19
coo
14
Transcribed Image Text:Question: Estimate the pl of the tripeptide ? Step 1: Determine what can be protonated/deprotonated Abundance Step 2: Estimate where the net charge of the protein would be = 0 Step 3: Use the pka's that bracket the neutral form. +H₂N COOH تیار کیا ہے CH₂ H CC H +1 H₂N H COOH CH3 CH3 0 1 2 cook CH₂ coof H pkg= 2.19 O H H₂N No charge H H соон CH3 CH3 3 4 pka= 3.86 Coo COO™ CH₂ H H H coof CH3 CH3 pka= 4.25 COO - 2 5 6 6 Coo J. CH₂ 7 pH O H H 8 H coo- CH3 CH3 COO" 9 + Н. H3N pka= 9.82 10 pka= 9.82 pka= 3.86 CH₂ H₂N 11 H H H coo CH₂ p - 3 O=C H H H 12 H pka= 4.25 O CH3 1 CH3 coo- CH3 CH3 COO 13 COO pka= 2.19 coo 14
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