Calculate protein concentration in unknown samples 1, 2, 3: Absorbance of Unknown 1 = 0.541 Absorbance of Unknown 2 = 0.85 Absorbance of Unknown 3 = 1.02 Standard Curve: Y = 0.0073x
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Calculate protein concentration in unknown samples 1, 2, 3:
Absorbance of Unknown 1 = 0.541
Absorbance of Unknown 2 = 0.85
Absorbance of Unknown 3 = 1.02
Standard Curve: Y = 0.0073x
![BSA (1.0)
Volume (ul)
1.0.0
2. 10.0
3.20.0
4. 25.0
5.30.0
6. 40.0
7. 56.6
[BSA]
Cugimi)
10
20
25
30
५०
50
x = long x 10μl
1ml
x=10µg/mL
Absorbance
595 nm
0.371
0.559
0.707
0.902
0.948
1.036
1.253
Net abs
595 nm
-
0.188
0.336
0.531
0.577
0.665
0.882
(sample-blank)
(0.707-0.371)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F790f8b68-f3fd-4a91-a49b-9e15d31eab3c%2F708364f9-fff0-40bf-a692-4a15f6e2c7fd%2Flnyydr_processed.jpeg&w=3840&q=75)
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