Question 3 ( How is an unknown sample identified using TLC? Mark all that apply. By comparing solubility of the sample to those of known compounds By comparing melting point of the sample to those of known compounds By comparing the Rf value of unknown to those of known compounds By comparing the color, shape, and distance traveled by sample to those of known compounds Question 4 (₁ IIC) What is the purpose of performing a thin layer chromatography experiment? Mark all that apply. To use TLC as a tool in identifying an unknown To use TLC as a tool to determine purity of compounds separated by extraction To identify aldehydes and ketones To separate components of a mixture
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- A flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?Explain the color changes observed throughout the peroxide value of oil experiment. Materials:1. Old and fresh oil samples2. Distilled water3. Acetic acid/chloroform solution4. Saturated potassium iodide solution5. Sodium thiosulphate (0.01M)6. 1% Starch indicator: dissolve 1g of starch in 10 ml of water and then add 90 ml of hotwater to itRf Sample values 0.21 В 0.39 C 0.33 0.27 Four amino acid samples were subjected to a normal-phase chromatography set up yielded the following Rf values. Which among the samples is the least polar? E and O A O B OD
- Sample contains (A, B, C, D) analyzed by thin layer chromatography using solvent system X the result showed: Rf Values: (A: 0.6, B: 0.8, C: 0.2, D: remained in the base line).1) If the sample was separated by using column chromatography. Which of these substances eluted first? And what is the detecting method? 2) If the sample was separated by using HPLC (1.Normal phase 2. Reversed phase) which RT of these substances comes first of both phases? And what is the meaning of RT? *In kool aid chromatography, what factors affect the efficiency of the column? Also discuss the Van-Deemter equation.A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.4 E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5.9 E-17 mol/[cell◦s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?
- Explain how acetaminopen gives such spectroscopic and chromatographic graph by interpreting the peaks that appeared in the graphs. Fig1. Mass Spectrometric Chromatogram of Acetaminophen (the two graphs) Fig2. IR Spectrograph of Acetaminophenpoints) You have to make 50mL of 0.05M HCI from a 10M HCI stock solution, but the onlymeasuring devices available are 100mL graduated cylinders and 10mL serological pipes. Howcould you accurately make the dilute HCI solution (clearly explain)?For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. 7. If the red cell suspension is the stock solution, what is being quantified in this test? What diluent/reactant should be used to detect your answer in number 7?
- Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…Nursing question MD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per milliliter of morphine SO4. How many milliliters of the stock solution is required to fill the Rx?A student performs a separation by TLC on a silica get plate that results in three spots. Ared spot is measured 23 mm away from the original sample spot, a blue spot is 42 mmaway, and a yellow spot is 49 mm away. The distance from the original spot to where thesolvent stopped is 55 mm.a) Calculate the Rf value for each component in the mixture. (Show your work and reportthe correct number of significant figures.) b) The silica gel plate is polar. Rank the three components in the mixture from lowest tohighest polarity. Explain your answer.