Answered: Problem 3. In the figure below, label…

Problem 3. In the figure below, label the following CRISPR-Cas9 components: Cas9, tracrRNA, crRNA, PAM site, spacer, protospacer, complementary strand, noncomplementary strand, RuvC, HNH

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter16: Reproductive Technology, Genetic Testing, And Gene Therapy

Section: Chapter Questions

Problem 13QP: The prospect of using gene therapy to alleviate genetic conditions is still a vision of the future....

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Concept explainers

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.

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Problem 3. In the figure below, label the following CRISPR-Cas9 components: Cas9, tracrRNA, crRNA, PAM site,
spacer, protospacer, complementary strand, noncomplementary strand, RuvC, HNH
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Hello, is this correct? What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a gene? Check All That Apply Cas1 geneCas1 gene Cas9 geneCas9 gene sgRNAsgRNA donor DNAdonor DNA bacteriophage DNA
CRISPR-Cas9 provides an unprecedented means of modifying the genome of almost any organism. Which of the following component(s) of this system contribute to the specificity of the endonucleolytic cleavage Options: the sequence of the sgRNA the PAM sequence Cas9 a and b a, b and c
n your experiment there will be 4 different experimental samples labeled A-D (please see Table 3 on page 12 of the Lab 4 handout). In sample D, if you saw some bacterial colonies that are blue and some colonies that are white, what would this tell you? Group of answer choices the sgRNA was completely non functional the CRISPR/Cas9 donor DNA repair is not 100% efficient there is no functional lacZ on this bacterial plate arabinose was not active on this bacterial plate
CA Live Remote Consider the following chart: What type of mutation is this? * DNA: TAC GCA TGG AAT MRNA: AUG CGU ACC UUA Amino acids: Met-Arg-Thr-Leu DNA: TAC GTA TGG AAT MRNA: AUG CAU ACC UUA Amino acids: Met - His - Thr- Leu substitution deletion insertion frameshift mutation
32. CRISPR #2: The CASCADE-DNA structure reveals the configuration of the crRNA- DNA duplex but it does not show how the subsequent nuclease DNA degradation step occurs. This DNA degradation step requires the Cas3 helicase-endonuclease enzyme. Cas3 is subsequently able to unwind and degrade the invading DNA. How does Cas3 specifically target the invading DNA?
4) List 3 things that a potential vector must have in order to be useful. 5) Sometimes the gene for the enzyme glutathione-S-transferase (GST) is used in purifying your protein of interest. Briefly explain how this is done. 6) What enzyme is used to create CDNA from MRNA?
Explain your experimental approach if you must correct the genetic defect phenylketonuria, which is caused by mutations in the phenylalanine hydroxylase gene using the CRISPR/Cas9 system and homology directed DNA repair.
Which of the following statements about the CRISPR-Cas9 technique is correct? 1. Catalytically inactive Cas-9 (dCas9) cuts only one strand of DNA. 2. The guide RNA used in the lab is analogous to the tracrRNA/crRNA hybrid in the adaptive immune system of bacteria. 3. Homology directed repair has a high degree of efficiency (ie, most target regions show integration of donor DNA). 4. NHEJ can lead to mutations. O A. 1, 2 and 3 B. 1 and 3 C. 2 and 4 D. 4 only E. All of 1, 2, 3 and 4 are correct O
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This is a multiple answers question. Which statement is true about CRISPR-Cas system? CRISPRS stand for clustered regularly interspaced short palindromic repeats Both bacteria and archaea can protect themselves from viral infection by using this system. Cas protein and short RNA form a complex The CRISPR region of DNA contains DNAS from previous infection by phages. Cas protein cuts target foreign DNA in pieces. The short RNA in the Cas protein and RNA complex has no function.