Description of how you have carried out aseptic techniques to cultivate these microorganisms to obtain pure cultures of bulgaricus and S. thermophilus from the mixed population (explain the principles behind each aseptic techniques used) and confirmation of these bacterial species by performing Gram’s staining.

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter15: Instrumentation In Medical Imaging
Section: Chapter Questions
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 Activity 2

 You are required to culture and identified yogurt starter cultures, Lactobacillus bulgaricus and Streptococcus thermophilus from a diluted yogurt sample (1ml of yogurt was dissolved in 9ml of peptone water) and observed the growth rates of Streptococcus thermophilus under varying conditions. The standard media accepted by the International Dairy Federation of the yogurt species, L. bulgaricus and S. thermophilus are MRS and M17 agar, respectively.

 You are also required to carry out different staining and biochemical techniques to identify different medically important bacterial species apart from yogurt cultures.

 

 Produce a report that includes;

  • Description of how you have carried out aseptic techniques to cultivate these microorganisms to obtain pure cultures of bulgaricus and S. thermophilus from the mixed population (explain the principles behind each aseptic techniques used) and confirmation of these bacterial species by performing Gram’s staining.
  • Description of how you have modified this experiment to see the effect of temperature and pH on the thermophilus growth and the techniques you have used to measure the growth of S. thermophilus?
  • Justify how the survival of yogurt cultures used to increase the quality of dairy products.
  • Describe how specimen collection is performed using aseptic techniques to isolate medically significant bacteria and identify these clinically important bacterial species, Escherichia coli, Streptococcus pneumonia, Haemophilus influenzae and Streptococcus pyogenes by using different staining techniques and biochemical tests. Evaluate the effectiveness of different biochemical techniques used to identify different bacterial species.

 

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