Part F - Identifying conserved residues from the BLAST alignment If an amino acid is found at the exact same location in the alignment of two homologous proteins, that amino acid is said to be "absolutely conserved." An amino acid type can also be conserved at a given position. Using the BLAST sequence alignment above, sort aligned residues into the appropriate categories. Drag the appropriate alignments to their respective bins. ► View Available Hint(s) I→L Q→E V→Q D→S Absolutely conserved W F T→M D→N Conserved by type N→G K→K A→E Not conserved Reset Help

AAAGAGAAAAGAAUA to AAAGAGAAAUGAAUA. Suppose the codon sequence has a single base pair mutation If the old protein sequence was Lys-Glu-Lys-Arg-Ile, what will be the new sequence encoded by the mutant gene? (Use the 3-letter amino acid abbreviations with hyphens and no spaces in between, i.e. Ser-Asn-Tyr-Leu-Pro.) Submit Answer Retry Entire Group No more group attempts remain

uestion 12 of 30 > Attempt 2 5'-GGU-3',5'-AAG-3',5'-IAC-3',5'-UGU-3', and 5'-CAA-3'. Give all A series of tRNAs have the anticodons, possible codons with which each tRNA can pair. Consider the wobble rules listed in the table. First position of anticodon C G A U I Third position of codon G U or C U A or G A, U, or C 5'-GGU-3' 5'-AAG-3' 5'-IAC-3' 5'-UGU-3' 5'-CAA-3' 3'-UUU-5' 3'-GUU-5' 3'-UUC-5' 3'-GUG-5' 3'-UUG-5' Answer Bank 3'-ACA-5' 3'-AAA-5' 3'-CUG-5' 3'-GCA-5' 3'-CCA-5' 3'-ACG-5' 3'-UGU-5' 3'-CCC-5' 3'-UCA-5' 3'-AUG-5'

Do you expect a Pro → Gly mutation in a surface-loop region of a globular protein to be stabilizing or destabilizing? Assume the mutant folds toa native-like conformation. Explain your answer in terms of the predictedenthalpic and entropic effects of the mutation on the ∆G for protein foldingcompared to ∆G of folding for the wild-type protein