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- The SARS-Cov-2 virus is the virus that cause COVID-19. The viral protease is a known target for finding potential viral inhibitor. The activity of the viral protease can be assayed using the synthetic peptide substrate attached to 7-amino-4-carbamoylmethylcoumarin (PACC). The viral protease cleaved the substrate producing the fluorogenic ACC which can be monitored using a spectrofluorometer. The following is data from SARS-Cov-2 protease (0.1 mg of enzyme) upon reaction with various concentrations of the substrate PACC. Plot the appropriate graphs to find: - The approximate Vmax from the Michaelis-Menten curve.Describe the molecule shown in the image. Evaluation at Atomic Resolution of the Role of Strain in Destabilizing the Temperature Sensitive T4 Lysozyme Mutant Arg96-->His(a) Answer all sections (i)- (Iv). Below is given the structure of three anti-HIV drugs: OH Meo. OMe NH Ph Nevirapine Atazanavir Staudivine (i) Give the drug target and discuss the anti-HIV mechanism of each of these drugs. Vaccines are an attractive prophylactic treatment against both viral and bacterial diseases. A number of glycaconjugate vaccines using bacterial polysaccharide structures conjugated to protein carriers have been introduced into mass vaccination schemes with excellent results. What differences are there in the immune response to a glycoconjugate vaccine as compared to a polysaccharide vaccine? These glycoconjugate va nes has been used for 30 years without any change in their structures, while the composition of the flu vaccine, based on attenuated or killed virus particies, is changed from year to year. Discuss the reason behind this. (iv) Discuss why it has not been possible to develop a (part structure) vaccine against the HIV virus, based on either I) viral…
- Which letter represents the target site of furosemide (Lasix)? E- -BIndicate whether each of the following events occurs when tryptophan is high or when tryptophan is low by placing a check in each of the appropriate blanks. Share your reasoning. Event Tryptophan high Tryptophan low Ribosome does not stall at Trp codons ____________ ____________ Region 2 of the leader pairs with region 3 ____________ ____________ Ribosome covers part of region 2 of leader ____________ ____________ Transcription is terminated before structural genes are transcribed ____________ ____________What is the expansion of acronym MICA?
- Among the following structures, the drug that can be given orally against B-lactamase-producing strain: is: CO-H OCH COOH I COOH II NH2 HO COOH III IV COOH Oll ONone of the above OlI OIVWild type SSL2 reference sequence from amino acid 621 through 680 621 Lys Met Gly Lys Pro Phe Ile Tyr Gly Ser Thr Pro Gln Gln Glu Arg Met Asn Ile Leu 640 1861 AAA ATG GGA AAA CCA TTT ATT TAT GGT TCC ACA CCA CAA CAA GAG CGT ATG AAC ATT CTG 1920 641 Gln Asn Phe Gln Tyr Asn Asp Gln Ile Asn Thr Ile Phe Leu Ser Lys Val Gly Asp Thr 660 1921 CAA AAT TTC CAG TAC AAT GAC CAA ATC AAT ACC ATA TTT CTA TCA AAA GTT GGT GAT ACT 1980 661 Ser Ile Asp Leu Pro Glu Ala Thr Cys Leu Ile Gln Ile Ser Ser His Tyr Gly Ser Arg 680 1981 TCC ATC GAT TTA CCA GAA GCT ACC TGT TTA ATT CAA ATA TCT TCG CAC TAT GGG TCT CGT 2040 Candidate mutant SSL2 sequence from amino acid 621 through 680 621 Lys Met Gly Lys Pro Phe Ile Tyr Gly Ser Thr Pro Gln Gln Glu Arg Met Asn Ile Leu 1861 AAA ATG GGA AAA CCA TTT ATT TAT GGT TCC ACA CCA CAA CAA GAG CGT ATG AAC ATT CTG 641 Arg Asn Phe Gln Tyr Asn Asp Gln Ile Asn Thr Ile Phe Leu Ser Lys Val Gly Asp Thr 1921 CGA AAT TTC CAG TAC AAT GAC CAA ATC AAT ACC ATA TTT CTA TCA AAA GTT GGT GAT ACT…Proteinase K should be used as the first step of DNA purification (before applying phenol CIA mixture) to remove most proteins. Proteinase K alone cannot remove all proteins from the lysate. Explain why the Proteinase K enzyme cannot remove ALL proteins from the cell lysate.
- Give typing answer with explanation and conclusion The following gene codes for a liver enzyme. Changing the A/T base pair to which of the following would result in a missense mutation? 5’ GCCTAATATGCCCGTATGC(AGC)nCGAATAAAATAGTACGGTCGTCGC 3’ 3’ CGGATTATACGGGCATACG(TCG)nGCTTATTTT ATCATGCCAGCAGCG5’ A. C/G B. T/A C. G/C D. Any of the above E. 2 of the above F. None of the aboveA sample of an unknown peptide was divided into two aliquots. One aliquot was treated with trypsin, and the other with cyanogen bromide. Given the following sequences of the resulting fragments, deduce the sequence of the original peptide. Trypsin treatment: Asn-Thr-Trp-Met-Ile-Lys Gly-Tyr-Met-Gln-Phe Val-Leu-Gly-Met-Ser-Arg Cyanogen Bromide treatment: Gln-Phe Ile-Lys-Gly-Tyr-Met Ser-Arg-Asn-Thr-Trp-MetSecond letter A UUU UCU) UC UCA UCG UAU UUC Phe UUA Tyr UGU UGCJ Ser UAA Stop UGA Stop A UAG Stop UGG Trp UUG Leu CAUHIS CUU CUC CUA CUG CCU* C ССА CCG CGU His САС Leu CGC Arg CGA Pro CAA Gin CGGJ Gln Which amino acid is carried by the TRNA with the anticodon 5'-UCA-3? ACU ACC ACA AAU AAC. AGU AGC AGA AUU Ser Asn AUC Ile A AUA Thr AAA Lys AAG Lys AGG Arg AUG Met ACG GAU GGU] GUU GUC GUA GUGJ GCU GCC GCA GCG GAC Asp Ala GAA GGC Gly GGA Val GAG Glu GGGJ Isoleucine. None-this is a stop codon. Aspartic acid. Histidine. IV. Leucine O V. Third letter UCAG UCAG UCAG First letter