Measuring gene expression of a reporter gene (e.g. gfp) under the control of an unknown promoter could be used to?

Q: E30. An electrophoretic mobility shift assay can be used to study the binding of proteins to a…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Tỏ idéntify transcriptional regulatory elements, reporters containing 100 base pairs of Promoter…

A: Reporter genes help us to understand and determine the expression of gene of interest by fusing them…

Q: An electrophoretic mobility shift assay can be used to study the binding of proteins to a segment of…

A: Question - An electrophoretic mobility shift assay can be used to study the binding of proteins to a…

Q: Measuring gene expression of a reporter gene (e.g. gfp) under the control of an unknown promoter…

A: A reporter gene is a gene that is stuck to a regulatory sequence (promoter) of the gene of interest…

Q: Transformation is a process in which bacteria take up new DNA released by dead cells and integrate…

A: An operon is defined as a group of genes that have a common promoter and regulator and also…

Q: Propose a strategy to prevent HIF-alpha signaling in the TME. What do you think would happen in a…

A: The oxygen-sensitive transcriptional activator HIF-1 (hypoxia-inducible factor-1) is an important…

Q: Q.No.6 Northern blotting is used to determine the amount and size of a particular RNA transcribed in…

A: Among a mixture of diverse RNA, through the northern blot transfer technique, specific RNA sequences…

Q: Write down transcriptional level control and translation level control. Explain with logic which…

A: Deoxyribonucleic acid, abbreviated as DNA, is a double-helix made-up of nucleic acids. Its role in…

Q: Why would the biosynthetic enzymes of a cell be repressible rather than constitutive or inducible?

A: Enzymes can be constitutive, inducible, or repressible. A repressor blocks transcription when it…

Q: Regulatory regions for genes can also be cloned using molecular-genetic techniques, allowing…

A: Yes, Regulatory regions of genes can be cloned using molecular genetic techniques for studying gene…

Q: Yes or no? is siRNA result of activity of argonaut? Does RNAi result permanently heritable in…

A: Yes, Argonaute proteins are the active part of RNA-induced silencing complex, cleaving the target…

Q: You isolated a gene expressed in differentiated neurons in mice. You then fused various fragments of…

A: ANSWER;- The Promoter is a little DNA (deoxyribonucleic acid) arrangement of a gene, where the RNA…

Q: During experimental RNAi, how does the researcher affect expression of a target gene? Group of…

A: Introduction: In the process of RNA interference(RNAi) double-stranded RNA molecule suppresses the…

Q: Propose some specific uses of a modified CRISPR-Cas system as a general RNA-guided device for…

A: CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas is a gene-editing tool. It…

Q: . Describe which enzymes are required for lactose and tryptophan metabolism in bacteria when lactose…

A: ANS 1.The enzymes of the lactose operon are needed to break down and use lactose as an energy…

Q: B. Briefly describe how can RNA seq be used to quantify differential gene expression between two…

A: It is attainable by performing RNA-Seq with spike-ins, samples of ribonucleic acid at identified…

Q: Microarrays can be used to determine relative levels of gene expression. In one type of microarray,…

A: Antibiotic resistance causes growth of various strains of bacteria even after the addition of…

Q: You construct a translational reporter gene fusion of your favourite gene. How can you assess…

A: Translational Gene Fusion is employed to live protein localization by quantifying promoter activity.…

Q: a. How many enhancers were you able to identify with these set of experiments? Explain. b. If you…

A: Enhancers are the term used for the regions of DNA (deoxyribonucleic acid) that elevate the…

Q: Q21. What regulators of gene expression bind the lac promoter region if E. coli is grown in media…

A: In the absence of lactose the repressor binds to the operator sequence adjacent to the promoter and…

Q: Can expression or the timing of expression of therapeuticgenes be controlled in a patient so that…

A: In genetics, gene therapy is defined as the introduction of the genetic material  in the DNA in…

Q: Q. Promoters and enhancers are important regulatory sequences for many eukaryotic protein-coding…

A: Transcription is a process of formation of transcript or RNA from DNA by the process of…

Q: Q34. mRNA decay (breakdown) can play an important role in controlling protein abundance. Which of…

A: mRNA decay or break down takes place in our cell when specific mRNAs are not required anymore and is…

Q: Q28. Which of the following is a sequence that would be likely to be bound by a transcription…

A: Transcription factor: To initiate transcription, eukaryotic RNA polymerase requires the help of…

Q: Explain how HOTAIR plays a role in the transcriptional regulationof particular genes.

A: A gene is a sequence of nucleotides in genome that codes for a functioning molecule. There is…

Q: Provide a detailed description of gene expression and control in prokaryotes. Provide a detailed…

A: Gene expression is the mechanism by which information from a gene is used to create a functioning…

Q: Explain the features of the Initiator (Inr) elements, BREs, DPEs, and MTEs of focused promoters.

A: BASIC INFORMATION  TRANSCRIPTION  it is responsible for the formation of hnRNA which has the codes…

Q: What is a promoter? A region of RNA on a transcript, upstream of an ORF, in which the RNA sequence…

A: Promoter is the DNA gene part on which the RNA polymerase binds and starts the process of…

Q: he steps to replicate the identified spike protein are as follows: . Find the CDNA sequence of the…

A: Gene expression is a phenomenon in which gene is expressed into a particular protein. It is a very…

Q: Gene Expression

A: Introduction  The Gene Expression Omnibus (GEO) data set is a worldwide public store that documents…

Q: Research indicates that promoters may fall into one of two classes: focused or dispersed. How do…

A: The promoters are the regulator of the ‘Gene Transcription’ process. The eukaryotic promoters are…

Q: Q.) A.)Search in human genome if any examples of mRNA translated from 2 different sites?and give…

A: The process of translation is the process of gene expression. This process is characterized by the…

Q: When dsRNA is treated with Dicer enzyme siRNA and miRNA’s are produced. What role they will play in…

A: siRNA is a type of double stranded short sequence rna which is non coding and take part in RNA…

Q: Promoters and enhancers are important regulatory sequences for many eukaryotic protein- genes. Which…

A: Transcription is the process of forming transcripts or RNA from DNA through complementary base…

Q: Yes or no only. rna seq can provide sequence and expression data   do riboprobes synthesize bu in…

A: Rna seq can provide sequence and expression data   : YES  Single-cell RNA sequencing (scRNA-Seq)…

Q: NCREASED/DECREASED transcription of target genes. Select one: O a. Decreased ; Decreased O b.…

A: GTPase-Activating Proteins or GAPs act by binding to the GTPase. This enhances the hydrolysis of…

Q: Compare the auto induction media (AIM) with regular induced protein expression. Which is better when…

A: Most of the eukaryotic proteins are still produced via genetic engineering it into host prokaryotic…

Q: 1. Draw a simplified model of a cis-regulatory element with multiple trans-acting factors…

A: A double-stranded DNA contains two regions, coding and non-coding. The non-coding region contains…

Q: how have microarrays demonstrated that, all cells of an organism have the same genome, some genes…

A: Lab-on-a-chip is another name for microarray. This method is used to simultaneously evaluate the…

Q: For each of the ff. scenario, state whether the gene is up- or down-regulated and briefly explain…

A: Up-regulation of gene expression refers to the increased expression of a gene by increasing the…

Q: Compare the actions of positive and negativeregulatory proteins at promoters

A: Lac operon is an operon that involves in the transport and metabolism of lactose in humans and many…

Q: Describe which enzymes are required for lactose and tryptophan metabolism in bacteria when lactose…

A: Tryptophan is aa that is required for proper newborn growth as well as the formation and maintenance…

Q: Match whether the protein binds directly or indirectly (assuming it does) to DNA and whether to the…

A: Enhancers are commonly known as cis-acting DNA sequences that can boost gene transcription. To begin…

Q: If you were given a tube containing only a purified protein of interest but had unlimited time and…

A: Every cell of a multicellular organism or a single-celled organism functions fundamentally with the…

Q: Expression of a gene, in terms of greater production of the protein it encodes, could be increased…

A: Gene expression is a process of formation of functional gene products from genes through…

Q: DA Different response elements located upstream of the promoter of the prokaryotic and eukaryotic…

A: Response elements are the portion of the gene with a short sequence of DNA within the promoter or…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.