. One mechanism by which antisense RNAs act as negative regulators of gene expression is by base pairingwith the ribosome binding site on the sense mRNA toblock translation. In a second, alternative mechanism,the act of transcribing an antisense RNA can somehow prevent RNA polymerase from recognizing thesense promoter for the same gene. Design an experimental approach that would enable you to distinguishbetween these two modes of action at a specific gene.(Hint: What would be the outcome in each case ifhigh levels of the antisense RNA were transcribedfrom a gene on a plasmid?)

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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. One mechanism by which antisense RNAs act as negative regulators of gene expression is by base pairing
with the ribosome binding site on the sense mRNA to
block translation. In a second, alternative mechanism,
the act of transcribing an antisense RNA can somehow prevent RNA polymerase from recognizing the
sense promoter for the same gene. Design an experimental approach that would enable you to distinguish
between these two modes of action at a specific gene.
(Hint: What would be the outcome in each case if
high levels of the antisense RNA were transcribed
from a gene on a plasmid?)

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