Answered: It was suspected that the cause of the…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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It was suspected that the cause of the infertility seen in a patient was due to undiagnosed non- classic late onset congenital adrenal hyperplasia. In order to determine the presence of a specific mutation in the patient, PCR amplification of the CYP21 gene was undertaken on a DNA sample from patient. The amplified DNA was subsequently digested with a restriction enzyme (RE). A normal gene has four cut sites for the RE, however a gene with this specific mutation would show the loss of one of the cut sites.

After the DNA fragments have been separated by agarose gel electrophoresis, if the patient was heterozygous for the mutation, indicate how many fragments you would most likely expect to see on the gel.

Select ONE option:

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