iodine test for polysaccharides
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iodine test for polysaccharides
a.on the spot plate separately place 5 drops of 3 % solution of starch and glucose. to each spot, add 2 drops of iodine solution.
b.observe the color formed.
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- Complete the table below. Indicate what is observed if each test is positive. State what this result implies about each of the carbohydrates tested. TEST EXPECTED OBSERVATION FOR A POSITIVE TEST SUBSTANCE INDICATED BY A POSITIVE TEST I2 Solution Molisch Barfoed Seliwanoff Bial- OrcinolWhich of the different carbohydrate test would give a positive result for maltose? Describe the color change.Will disaccharides and polysaccharides give a positive result for Molisch test? Which of the different carbohydrate tests would give a positive result for maltose? Describe the color change.
- B. Test for Glucose color with Benedict's presence of Glucose (+ or -) test solution with reagent Starch Starch + AmylaseWhich of the carbohydrate tests (Molisch, Seliwanoff, Benedict, Barfoed, Bial, Iodine Tests) would be positive for maltose? Include description of the color change. Explain in 2-3 sentencesAn IV bag contains 250 mL of a 5% mannitol solution. How many grams of mannitol are in the IV bag?
- Will all carbohydrates gives a positive result with Molisch test? Explain your answer:Hydrolysis of polysaccharidesa. Add 20 drops of concentrated HCl to 30 mL of 3 % starch solution.Cover the beaker containing the mixture with a watch glass and boilgently.b. After one minute addition of acid solution, remove 2 drops of thesolution and place on a spot plate. Test each spot with one drop ofiodine solution. c. Perform the same test using Benedict’s solution instead of iodinesolution.Which of the different carbohydrate tests would give a positive result for maltose? Describe the color change. Molisch test Fehling’s test Benedict’s test Tollen’s Test Barfoed’s test Seliwanoff’s test Phenylhydrazine or osazone test
- Glycogen isolated from liver biopsy specimen had normal structure. Blood glucose level was below normal, uric acid and lipid levels were elevated. Write your diagnosis.Explain with pictures or drawing what are the lipid profile tests ( Cholesterol, Triglycerides, HDL and LDL).Hydrolysis of polysaccharidesa. Add 20 drops of concentrated HCl to 30 mL of 3 % starch solution. Cover the beaker containing the mixture with a watch glass and boil gently. b. After one minute addition of acid solution, remove 2 drops of the solution and place on a spot plate. Test each spot with one drop of iodine solution. c. Perform the same test using Benedict’s solution instead of iodine solution. Hydrolysis of polysaccharides Results Starch Solution Iodine's Test Benedict's Test