In the fluorescence quenching of rhodamine B dye, the fluorescence decay of rhodamine B in the DMF-solution with ethanol-water mixture was monitored by measuring the 605 nm emission lifetime (decay of intensity over time) after a laser pulse. The lifetime (t) of rhodamine B in absence of quenchers is Do 2.10 ns. Quenching of rhodamine fluorescence by the presence of 2% ethanol- water mixture reduced the lifetime (T) to 1.86 ns. Using the following Stern-Volmer equation, what would be the rhodamine B emission lifetime in ns by changing the ethanol-water to 13 %? (Final answer should be of three significant figures) 1.142 To T = k₁ + k₂+k₂ [Q] k₁ + k₁₂
In the fluorescence quenching of rhodamine B dye, the fluorescence decay of rhodamine B in the DMF-solution with ethanol-water mixture was monitored by measuring the 605 nm emission lifetime (decay of intensity over time) after a laser pulse. The lifetime (t) of rhodamine B in absence of quenchers is Do 2.10 ns. Quenching of rhodamine fluorescence by the presence of 2% ethanol- water mixture reduced the lifetime (T) to 1.86 ns. Using the following Stern-Volmer equation, what would be the rhodamine B emission lifetime in ns by changing the ethanol-water to 13 %? (Final answer should be of three significant figures) 1.142 To T = k₁ + k₂+k₂ [Q] k₁ + k₁₂
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter12: Atomic X-ray Spectrometry
Section: Chapter Questions
Problem 12.12QAP
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Question
![In the fluorescence quenching of rhodamine
B dye, the fluorescence decay of rhodamine
B in the DMF-solution with ethanol-water
mixture was monitored by measuring the
605 nm emission lifetime (decay of intensity
over time) after a laser pulse. The lifetime (t)
of rhodamine B in absence of quenchers is Do
2.10 ns. Quenching of rhodamine
fluorescence by the presence of 2% ethanol-
water mixture reduced the lifetime (T) to 1.86
ns. Using the following Stern-Volmer
equation, what would be the rhodamine B
emission lifetime in ns by changing the
ethanol-water to 13 %? (Final answer should
be of three significant figures)
1.142
To
T
=
k₁ + k₂+k₂ [Q]
k₁ + k₁₂](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F62ed44fe-998a-4717-8957-0cea65d35438%2F7a1b8823-8d20-4e68-b005-6bc2d9602453%2Fr41nq3v_processed.jpeg&w=3840&q=75)
Transcribed Image Text:In the fluorescence quenching of rhodamine
B dye, the fluorescence decay of rhodamine
B in the DMF-solution with ethanol-water
mixture was monitored by measuring the
605 nm emission lifetime (decay of intensity
over time) after a laser pulse. The lifetime (t)
of rhodamine B in absence of quenchers is Do
2.10 ns. Quenching of rhodamine
fluorescence by the presence of 2% ethanol-
water mixture reduced the lifetime (T) to 1.86
ns. Using the following Stern-Volmer
equation, what would be the rhodamine B
emission lifetime in ns by changing the
ethanol-water to 13 %? (Final answer should
be of three significant figures)
1.142
To
T
=
k₁ + k₂+k₂ [Q]
k₁ + k₁₂
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