Fluorescence from the energy donor pyrene isquenched by the acceptor dye coumarin. In an experimentto investigate the binding of pyrene within a substrate. thefluorescence quantum yield of pyrene in the absence of anyquencher was measured to be 0.65. W ith coumarin bound tothe surface of the substrate. the quantum yield dropped to0.49. Use Forster theory to calculate the separation betweenthe donor and acceptor. given that the value of R0 for thepyrene-coumarin pair is 3.9 nm.
Fluorescence from the energy donor pyrene isquenched by the acceptor dye coumarin. In an experimentto investigate the binding of pyrene within a substrate. thefluorescence quantum yield of pyrene in the absence of anyquencher was measured to be 0.65. W ith coumarin bound tothe surface of the substrate. the quantum yield dropped to0.49. Use Forster theory to calculate the separation betweenthe donor and acceptor. given that the value of R0 for thepyrene-coumarin pair is 3.9 nm.
Principles of Modern Chemistry
8th Edition
ISBN:9781305079113
Author:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Publisher:David W. Oxtoby, H. Pat Gillis, Laurie J. Butler
Chapter20: Molecular Spectroscopy And Photochemistry
Section: Chapter Questions
Problem 6P
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Fluorescence from the energy donor pyrene is
quenched by the acceptor dye coumarin. In an experiment
to investigate the binding of pyrene within a substrate. the
fluorescence quantum yield of pyrene in the absence of any
quencher was measured to be 0.65. W ith coumarin bound to
the surface of the substrate. the quantum yield dropped to
0.49. Use Forster theory to calculate the separation between
the donor and acceptor. given that the value of R0 for the
pyrene-coumarin pair is 3.9 nm.
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