Imagine after completing all steps for western blotting you see that your membrane is FULL of dark colored staining. Which of the following steps was MOST LIKELY the cause? No primary or secondary antibody actually added O Secondary antibody not binding 5% milk blocking solution Primary antibody not binding Each strain of Neurosporo used had at least one mutation/deletion present. All of these mutations/deletions are found in genes related to: Cell wall associated proteins, Molecular motor proteins O Ubiquitin proteins Nuclear envelope proteins

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
icon
Related questions
Question

Please answer fast

 

Imagine after completing all steps for western blotting you see that your membrane is FULL of dark colored staining. Which of the following steps was MOST LIKELY the
cause?
No primary or secondary antibody actually added
Secondary antibody not binding
5% milk blocking solution
Primary antibody not binding
Each strain of Neurospora used had at least one mutation/deletion present. All of these mutations/deletions are found in genes related to:
Cell wall associated proteins
Molecular motor proteins
Ubiquitin proteins
Nuclear envelope proteins
Transcribed Image Text:Imagine after completing all steps for western blotting you see that your membrane is FULL of dark colored staining. Which of the following steps was MOST LIKELY the cause? No primary or secondary antibody actually added Secondary antibody not binding 5% milk blocking solution Primary antibody not binding Each strain of Neurospora used had at least one mutation/deletion present. All of these mutations/deletions are found in genes related to: Cell wall associated proteins Molecular motor proteins Ubiquitin proteins Nuclear envelope proteins
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Types of communication
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education