50 please answer all questions Find the connection among the words below and choose the letter of theword which is different.A. Gel docB. Ethidium bromideC. UV lightD. transilluminatorFind the connection among the words below and choose the letter of theword which is different.OA. PCR productB. DNAC. wellsO D. tracking dye

Gel doc is an equipment most commonly used in molecular biology lab procedures for the visualisation…

Answered: Find the connection among the words…

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 4RQ: What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel...

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Which of the following techniques separate molecules by size using electric charge? a. Group of answer choices b. Gel electrophoresis c. PCR d. restriction enzymes gel filtering
PCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleases
The Tm of primers is critical to PCR design. For the primers belowwhich has the lower Tm ? Why? Choose one primer and one reason a.CGATOGTACGATCGTAGCATCGAb. TGO CGATGCTACGAT c. The primer is longer, therefore the Tm is lower due hydrogen bonds. d. The GC is higher (has more GC base pairs ) therefore the TM is lower due more hydrogen e. The AT content is higher has more AT )the Tm is lower due to more hydrogen bonds f. The primer is shorter and therefore has fewer hydrogen bonds
What are the advantages of qPCR (RT-PCR) compared to conventional PCR? Choose all that apply a. human error is reduced as there are fewer human interactions with the samples b. you can visualize the results as the process is running c. samples can be compared as to the amount of template DNA in the original sample d. more samples can be run in a day by one person
1. You are working as a CSI analyst. You collected DNA at a crime scene that is from the suspect but do not have enough to run a test to determine who the suspect is. Please discuss what you would do to attain more of the DNA and how to test the DNA to determine who the suspect is.2. Please discuss how the bacterium Agrobacterium tumefaciens normally functions. Please also discuss how this bacterium can be used to help plants attain genes to help fight off insects and herbicides.
All are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚C
During real-time PCR, the synthesis of PCR products is analyzeda. at the very end of the reaction by gel electrophoresis.b. at the very end of the reaction by fluorescence that is emittedwithin the thermocycler.c. during the PCR cycles by gel electrophoresis.d. during the PCR cycles by fluorescence that is emitted withinthe thermocycler.
Choose the basic chemical materials that are required to perform PCR. A. Two oligonucleotide primers. B. DNA fragment to be amplified. CA heat-resistant DNA polymerase. D. All of the above
Define and identify the words listed below: 1. restriction enzyme 2. topoisomerase 3. PCR
Explain how electrophoresis separates DNA strands. a. How is a DNA fingerprinting test interpreted? b. Define plasmid and how plasmids can change a bacteria’s activity. c. How do we digest/cleave plasmids? Explain the role of a restriction enzyme. d. Define sticky end and blunt end and which one is useful in molecular biology.
Which of the following reagents is NOT required for a PCR? a. DNA template b. DNA Polymerase c. All four dNTPs d. small amount of ddNTPs e. Pair of primers
Polymerase chain reaction (PCR) carries out DNA replication in a test tube. Which of the following is NOT used in a PCR reaction? A. DNA polymerase B. Primase OC. DNA nucleotides OD. Template DNA

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