Explain why these two DNA samples give different results, when they're both 50% G-C. Fraction of DNA 1 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 ---E coli DNA - Potoroo DNA
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- True or False, if the statement is false, provide the correct answer. 1. The amino acid sequence is not responsible for specifying the 3D shape of a protein. 2. The red, orange, and yellow pigments found in plants, that are often visible in the fall are chlorophyll. 3. There are 10 base pairs in one full turn of the DNA double helix. 4. The stroma is a gel-like material within the chloroplast that corresponds to the cytoplasm of the original bacterium. 5. Plants gather the sun's energy with light-absorbing molecules called pigments.A 918,980 Å long double stranded DNA molecule is the B-conformation. If the DNA has has alternating GC Sequence and adopts the Z-conformation. How long is it now?1 2 3 4 (kbp) (bp) 4. To the right is an image of an agarose gel. Lanes 1 and 4 are DNA markers (DNA sizes are known) and serve as a reference to estimate the size of DNA fragment of interest in Lane 2 and 3. 4.36 2.32 a. What is the approximate size of the fragment in lane 2? Pay attention to units! 500 -200 b. What is the approximate size of the fragment in lane 3 (near the bottom)? Pay attention to units!
- HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. After 4 minutes in the blender, what percentage of each isotope was extracellular?HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. How did the researchers know that the radioisotopes in the fluid came from outside of the bacterial cells and not from bacteria that had been broken apart by whirling in the blender?HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. The extracellular concentration of which isotope increased the most with blending?
- HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. Before blending what percentage of each isotope. 35S and 32P, was extracellular (outside the bacteria)?HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. Do these results imply that viruses inject DNA or protein into bacteria? Why or why not?Demuestra lo que sabes del vo X ure.com/courses/57129/quizzes/206819/take Question I8 1 pts 3.7- Chargaff's Rule & The Hayflick Limit In 1950, Erwin Chargaff analyzed the base pair composition of DNA. He discovered that... % Adenine = % Thymine % Cytosine = % Guanine Meaning... There are equal amounts of Adenine and Thymine and equal amounts of Cytosine and Guanine in any given DNA sample. Question: Which of these statements is TRUE if there is 40% Guanine in a strand of DNA? There is 20% Adenine O There is 15% Thymine O There is 10% Cytocine O There is 10% Adenine
- researcher runs an agarose gel IIl ladder one läñé ând 100 ng of a 500 bp DNA fragment in another lane. The gel is 0.5% agarose, and it is run for 45 minutes time at 200 V. When he checked the gel, he saw three bands in the Hind III lane and no other bands on the gel. What can be the reason behind this. What additional information we may need to be sure about the reason.In E. coli, Base excision repair (BER) handles non-bulky lesions (e.g., deamination, oxidation) and nucleotide excision repair (NER) handles bulky lesions (e.g., Py dimers). Is this statement true or false? O True FalseO Off target effects are not really a concern. Question 20 What happens after a double stranded break is induced in the DNA? Select the statement that is FALSE. O HR which will lead to a small indel if template DNA is absent O Microhomology-Mediated End Joining O Non-Homologous End Joining O HR if template DNA is present Question 21 See below for four STR profiles from four different boys, as depicted in an electropherogram. The peak local