Explain how to conduct an investigation to extract DNA from a specimen of an animal Make sure to explain each major step and what the function of that step is.
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- Aside from the sample and assuming proper AGE protocol, which of the following is most needed to determine the DNA quantity using the eyeball method? loading buffer DNA ladder positive control negative controlDNA Cheek Cells Lab Identify the component of the solution which is the extracted DNABriefly explain the functions of the four procedures you learned about in this lab: DNA extraction PCR Gel electrophoresis DNA sequencing and analysis
- show the findings (DNA extraction-gel band; NO/YES) and briefly explain the interpretation of the resultsExplain the basis for identification using DNA fingerprinting. – relate this to Microbiology not to human fingerprinting.Why does this technique work? Mention restriction enzymes and their function. Keep answer between 2-3 sentencesExplain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to address the role of Taq DNA polymerase.
- Explain the difference between a DNA profile and a microrarray.DNA EXTRACTION Materials: knife 1 cup of fruit Table salt clean piece of cloth or strainer for filtering resealable bag dishwashing liquid 70% rubbing alcohol (chilled) shot glass (or any transparent and narrow container resembling a test tube) How to do the extraction: Before you begin, make sure you have chilled your alcohol in the freezer for at least 3 hours. Room temperature alcohol will not work nearly as well as cold alcohol. Place about 50 mL of alcohol in the freezer to chill it and take it out only when you’re going to need it. (The alcohol will not actually freeze.) 1. The first thing you will need is a sample. Since DNA is found in all living…Explain the basis for identification using DNA fingerprinting. – relate this to Microbiology not to human fingerprinting. Why does this technique work? Mention restriction enzymes and their function.
- A colleague wishes to separate a mixture of DNA fragments using gel electrophoresis and asks you if he can use alternating instead of direct current, because he has mislaid the DC equipment. How would you answer? Provide evidence.sample containing at least one molecule of DNA serves as the starting material for PCR. True Falseschematic diagram for the procedure in doing DNA extraction, isolation and quantification