In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and
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In your opinion, for efficient multiphoton excited fluorescence microscopy, would it be better to use a 100 fs 80 MHz laser pulse train or a 200 fs 80 MHz laser pulse train for imaging biological samples? Please explain your answer using equations 1 and 2
<I(t)2> = gP <I(t)>2 / (Rτ) ..............equa (1)
τout = τin (1 + 7.68(D/τ2in)2) 1⁄2 ................equa (2)
D is the total dispersion in femtoseconds squared
gp is a unitless factor that depends on the temporal laser pulse shape (0.66 for a Gaussian pulse shape), τ is the full-width half-maximum (FWHM) of time average value
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