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What are the possible reasons why culture plates may have too many colonies despite performing serial dilutions and OD600 readings?
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- Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000What is the goal of isolation streak plate technique? Why is microbial considered a pure culture?What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?
- Why is the looping out method preferable for sputum specimen for acid fast smears?Based on the found under #4 inoculating plates order, and the definitions above, what can you assume the order of plating cultures is based on? *What are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank you
- What are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage DisadvantageHow do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
- In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?What are the different manufacturing methods of Suppositories dosage form? Explain heat molding process using flow chart. Please explain at your own words.What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?