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1. Discuss how immunoassays differ from precipitation and agglutination reactions.
2. Differentiate between Direct and Indirect ELISA.
Step by step
Solved in 2 steps with 2 images
- 1.Explain the two types of ELISAs used. Be sure to explain how each test works, how they differ from each other, and when to use one type of ELISA versus the other. 2.How does Salmonella grow in host cells? How does it differ from Shigella? Be sure to explain pathogenic mechanism and how this correlates to symptoms.4. Immunoelectrophoresis tests: - What are pl and pH? - How to identify the pl and pH?state the significance of heterophile antibodies in serological testing
- 2. Reactions with labeled antibodies or antigens. Practical use of immunofluorescence reaction (IFR), enzyme-linked immunosorbent assay (ELISA), radioimmunoassay. Varieties of ELISA.a. Seropositivity means having a blood serum that tests positive. Explain why it may or may not develop at the same rate in all patients exposed to the same microbe. b. Would a high rate of false-positives decrease the sensitivity or specificity of a serological test?a. List the bacteria from this chapter for which general, routine vaccines are given. b. For which special groups of bacteria are there vaccines? c. For which bacteria is there none?
- 1. Give at least 2 serological tests used for infectious mononucleosis. 2. Differentiate Paul Bunnel from Davidsohn differential test.Describe the term serology and explain the immunologic principle behind serological tests.1. Answer the following: a. What is the significance of the three-dimensional shape (or geometry) of molecules in immunoassay methods? b. What is a radioimmunoassay (RIA) test in your own words.
- Compare and contrast Radial Immunodiffusion with it’s close relative, the Ouchterlony plate technique. How are they similar and how are they different?1. define the latex agglutination (LA) and gelatin particle agglutination tests (GPAT) techniques 2. State the principle underlying the above techniques 3. state the types or forms of the techniques if present1. State the principle underlying the latex agglutination (LA) and gelatin particle agglutination test (GPAT) techniques, and state the types or forms of the techniques if present.