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Create a schemetic diagram of the experimental procedures down below
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- Now prepare a 500 ml media having ¼ strength MS with 5mM BAP, 5.5 mg/l Kn and coconut water 10%. Stocks are MS Macro 10X, micro 20X, FeEDTA 10X, Organic 100X, BAP and Kn 5gm/100ml. If you need anything else then you add accordingly. BAP has a MW of 225.3. Sucrose and Myo-inositol is not mentioned in the question. These you need to add. Consistency of the medium is also mentioned.4. On a TLC plate containing silica gel, which compound will have the largest Rf value when ethyl acetate is used as the mobile phase? Circle your answer for credit. Compound A CH3 cor CH3 H₂C COOH Compound B H.C. 0 CH,. A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…
- 1. How much NaOH(in grams) is reguired to prepare 0.2 M of 250 m! NaOH?(Naoh Ma= 40g/mol) 2. AWhite blood cell Suspension is dyed by Trypan Blue in 1:1 Ratio(Volume). 7 square is counted and the values are; Total Viable Cells: 168 Total Nonviable Cells:32 a.Percentage of Viable Cells: ? b.Average Number Of cells per square: ? ç.Dilution Factor:? d.Concentration (Vable Cells/ml): ? 3. Amniotic fluid(AF) was taken from the mother for the baby with suspected disease. Cells were counted in the amniotic fluid, the sample was placed on the Thoma slide. There were too many cells. Only 6 randomly counted on the second size of square areas; Number of cells in this AF sample found 97, 123, 113, 72,60, 105. (100 µl Sample Dilluted with 200 µl Trypan Blue.) a. Average of cells = ? b. Number of cells in 1 ml=? 0,025 0,05 1 mm Figure 1: Area Number 1: x10.000; Area Number 2: x250.000.Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.THIN-LAYER CHROMATOGRAPHY 1. What is the purpose of placing a filter paper inside a beaker or developing chamber for TLC? 2. Among the three solvents used in the separation of pigments in spinach, which produced the most effective separation? Explain your choice. a. hexane b. 70:30 hexane:acetone c. 50:50 hexane:acetone 3. Research the pigments present on spinach leaves. Which among them correspond to each spot in the chromatogram resulting from TLC?
- other than 1. spheroplast technique 2. Heat denaturation, ammonium sulfate precipitation 3. ion-exchange chromatography on DEAE cellulose 4. SDS-page and Native Page is there another way to purify Alkaline phosphatase.6. You were asked by your laboratory instructor to prepare the following: 12 plates (use maximum volume) and 6 big slants of Malt Yeast Extract Glucose Agar. Calculate for the total volume of the media you need to prepare, and the amount (in grams) needed to be weighed for each component. Refer to the table below. Note: Show all computations. Use 3 significant figures for your final answer. Amount (per 200 ml) 0.6 g 0.6 g 4.0 g 3.4 g Malt Yeast Extract Glucose Agar Malt extract Yeast extract Glucose Peptone Agar 1.7 % Table 1. Glassware used in media preparation and their approximate volume capacity. Glassware Approximate volume* Petri dish 15-20 ml Big test tubes (18 mm diameter) as stabs/broth 10 ml as slants 6-8 ml Small tubes (13 mm diameter) as stabs/broth 5 ml as slants 3 ml It is prescribed not to exceed 60% of the total capacity of the container. Erlenmeyer flasks, media bottles, etc. *Volumes may vary depending on the laboratory practices.Composition of Cell Lysis Buffer Required Concentration of Reagent Molecular Weight Volume or Weight of Reagents Needed 40 mM Tris HCl 157.59 20 mM Acetic acid 60.05 1 mM EDTA 292.24 10 mM MgCl2 from 25mM stock 95.21 5% SDS 288.37 ddH2O 18.02
- 2. DECOLORIZATION Dissolve 1g of brown sugar in 15 ml. dist. Water in a small beaker and divide the solution into two equal portions. Place one portion in a test tube and add 1 g boneblack or activated charcoal and gently boil over a small flame for about 2 minutes (replace water lost by evaporation). Allow to cool and filter. How does the filtrate compare with the original solution? What property boneblack is exhibited in this procedure? How do you define this property? 3. CRYSTALLIZATION Mix in a beaker 2g of benzoic acid and 10ml of dist. Water. Boil gently over a flame for about 2 minutes, replacing water lost by evaporation every now and then. Filter the solution while still hot if any residue shows, if none, directly immerse the tube in a beaker of cold water (used ice water if available). Let stay for about 5 min. and observe. Are crystals formed? How do they compare with the original in texture in size of crystals? Define crystallization How can crystallization be carried? 4.…5. You were asked to prepare 250 ml of Acidified Potato Dextrose agar for the subculture of Alicyclobacillus sp. Given the composition of the medium below, compute for each component of Acidified PDA. :Components Amount per liter Potato Infusion 200.00 ml Dextrose 2.00% (w/v) Tartaric acid 1.00 ml of 10.00% solution (available as stock solution of 50,000 ppm) Agar 1.70% ‒‒‒‒‒‒‒‒‒‒‒‒‒ 5.3. Tartaric acid. Express your answers using two decimal places. Follow this format in typing your answers: answerabbreviated unit (e.g. 3.00 g. 0.75 ml, 10.25 mg) (2.5 pts).REMOVAL OF WATER FROM MICROBIAL CELLS USING FREEZE DRYING METHOD. Before starting the freeze-drying process, the weight of the test tube, Wi which is 15.33g and test tube with microbial cell pellets, Ww which is 34.94g were weighed using an electronic balance. Plus, final weight of the beaker with test tube and cells is 70.35g. Then, the average amount of water removal and average cell yield were calculated by using the formulas given which were 9.81g and 0.5001. As a conclusion, there was 9.81g of water had been removed by using the technique freeze-drying for 24 hours by using a freeze dryer. Question: 1.From this statement, please explain what process actually has happened during this freeze-drying method. 2.Make a clear conclusion that can made from this experiment.(explain briefly)