5. You were asked to prepare 250 ml of Acidified Potato Dextrose agar for the subculture of Alicyclobacillus sp Given the composition of the medium below, compute for each component of Acidified PDA. ▪▪▪▪▪▪▪▪▪▪ Components Amount per liter :Potato Infusion 200.00 ml Dextrose 2.00% (w/v) Tartaric acid 1.00 ml of 10.00% solution (available as stock solution of 50,000 ppm) Agar 1.70%
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- Consider the sterilization of a sodium gluconate production medium in the holding section of a continuous sterilizer. Assuming constant temperature, the specific death rate constant of the contaminant is 20 s-1 . If the average residence time in the holding section is 10 seconds, calculate the Del factor for the following and explain the results. i. For Pe = 400 ii. For Pe = 400, assuming plug flow iii. For Pe = 100 iv. For Pe = 100, assuming plug flowTime point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.N. benthamiana will be infiltrated with a solution containing OD600=0.3 of each experimental Agro containing construct and OD600=0.1 of p19. Calculate the volume of cultures (V construct; V p19) needed according to the formulas: V construct = V final × 0.3/OD600; V p19 = V final × 0.1/OD600. One mL of infiltrate is often enough to complete a small experiment. How to plan your final volume accordingly?
- A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ulAndrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:Sweet sorghum juice is used as sole carbon source for ethanol production by Saccharomyces cerevisiae. The overall in situ sterilisation duration is 40 minutes at 120 0C for a pilot reactor (50 L) which is filled with 60% of working volume. The non-sterile medium consists of 3.28 x 106 contaminants L-1. During the autoclaving process, you have noticed that the time taken for heating and cooling are 18 minutes and 15 minutes, respectively. As a process engineer, explain if you agree with this sterilisation practice by using quantitative analysis.
- Suspend 28.0 grams in the 1000 mL distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (1210C) for 15 minutes. Cool to 45-500C. If desired the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri dishes. 1. Determine the amount of dehydrated medium needed to prepare 50 nutrient agar plates. Include amount for 2 additional plates as excess to compensate for compounding losses.Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/LGIVE A DETAILED AND COMPLETE DESCRIPTION FOR THE FOLLOWING: 1. What are the culture media used in the following tests? — indole production test (indole production from tryptophan) — lysine decarboxylase test 2. What are the expected results in the following tests? — indole production test (indole production from tryptophan) — lysine decarboxylase test
- Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungido methodologies preparation of the following 6 solutions (Broth LB, LB Agar, Potato-Dextrose Agar, LB-Dextrose Broth, Saline solution 0.85%, Glycerol 80%). Include photos or schematics.As a public health engieer you have been tasked wiyh designing a plug flow reactor for the disinfection of Giardia lamblia using either free chlorine and monochloramine. The objective is a three log reduction in the concentration of Giardia lamblia. The kinetics of chlorine decay can be described by a first order coefficient of 0.1 d-1. It is also given that the appropriate contact time, t10, equals the hydrualic residence time (HRT or td) of the plug flow reactor. Question: At a temperature of 5oC and a pH of 7.0, what is the volume of the PFR if the flowrate of the water to be disinfected is 1000 L/d for chlorine and monochloramine Disinfection data in the table below can be used.