Compute for the appropriate amount (mL_) of the compositions of a CTAB NaCl solution for DNA extraction. a. 10% (w/v) CTAB b. 0.7 M NaCl
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Compute for the appropriate amount (mL_) of the compositions of a CTAB NaCl solution for DNA extraction.
a. 10% (w/v) CTAB
b. 0.7 M NaCl
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Solved in 2 steps
- Compute for the appropriate amount (mL) of the compostions below of TE buffer used for DNA extraction. a. 10 mM Tris b. 1 mM EDTAExpected 260/230 values for "pure" DNA are commonly in the range ofYou have PGFPuv at 75ng / uL You need 1.15 ng total DNA in a final volume of 30 . Calculate how much DNA and water you will need to add to both your Enzyme Mix and your Control Mix and fill out the table below.
- The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the initial flux of amino acids, give an instantaneous gradient of (20g/cm 3 )/8cm Why is polyacrylamide gel is used in electrophoresis?Various concentrations of recombinant human insulin were prepared for use standards for an HPLC method. To verify the prepared concentrations, the samples were analyzed by measuring the absorbance at 280 nm in a short path length (5 mm) cuvette. The molar absorption coefficient for human insulin is approximately 5.875 x 10³ M-¹cm-¹. a. Calculate the extinction coefficient in mL mg-¹cm-¹. b. Calculate the concentrations of the following human insulin standards if the measured absorbances and dilutions used are: Standard 1 Standard 2 Abs. (at 280 nm) of Diluted Sample 0.305 0.685 Dilution 145.0 µL sample, 25.0 μL buffer 130.0 µL sample, 40.0 μL bufferWhat is the concentration of a DNA solution with an OD260 measurement of 3.175 if 1OD unit at 260nm represents a DNA concentration of 50 g/mL? Write the correct units.
- Compute for the appropriate amount (mL_) of the compositions of a 10 mL 2x CTAB buffer for DNA extraction. Answer D and E a. 2% w/v CTAB b. 1.4 M sodium chloride c. 100 mM Tris-Cl d. 20 mM EDTA e. 2% polyvinylpyrrolidone MW 40 000A flat (not spherical) tissue engineered skin (thickness 2.5 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.4 E-9 m2/s, and according to experiments that you’ve performed, you want to make sure that the concentration of oxygen in the device doesn’t fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5.9 E-17 mol/[cell◦s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?The purification continues with a cation exchange step in which the positively charged cytochrome C protein is separated from negatively charged DNA and other proteins. The cation exchange eluate (volume of solution collected) had a total volume of 42.0 mL and a 1.0 mL aliquot was set aside for further analysis. The following data was obtained from the 1.0 mL aliquot to quantify the protein amount and purity: The absorbance at 410 nm of the aliquot was diluted 5-fold was 0.474 (1 cm pathlength). The absorbance at 595 nm from a 1.0 mL Bradford Assay solution that was diluted by 100-fold from the aliquot was 0.195 (1 cm pathlength). Using the information given, Calculate the total protein amount in mg from the absorbance at 595 nm. Calculate the cytochrome C amount in mg from the absorbance at 410 nm using Beer’s Law.
- A flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?The A280 of a protein sample loaded onto a gel was determined to be 0.767 (1.00 cm path length, after subtracting the blank). The total volume of this sample was 428 µL. 19.0 µL of this protein sample was mixed with 19.0 µL of 2X laemalli sample buffer and then 12.0 µL of the entire sample was loaded into the gel and electrophoresed. Calculate the amount of protein that was loaded into the gel (in µg).(A) Below is a partial purification table for a first attempt at a purification schedule for an enzyme: Technique Initial IMAC IEX Gel Filtration Protein (mg) 3467.8 196.7 153.6 142.1 Enzyme activity (units) 36489 32575 27891 26398 Overall yield (%) 100 Specific activity (units/mg) 29.46 Complete the table below by calculating the missing values. Overall Enrichment 1.00
