Various concentrations of recombinant human insulin were prepared for use standards for an HPLC method. To verify the prepared concentrations, the samples were analyzed by measuring the absorbance at 280 nm in a short path length (5 mm) cuvette. The molar absorption coefficient for human insulin is approximately 5.875 x 10³ M-¹cm-¹. a. Calculate the extinction coefficient in mL mg-¹cm-¹. b. Calculate the concentrations of the following human insulin standards if the measured absorbances and dilutions used are:
Q: the amino acid proline has a net negative charge at Ph of 12 true of false
A: The isoelectric point (pI) of an amino acid is the pH at which there is no net charge on the amino…
Q: What kind of protein is casein? POST-LABORATORY QUESTIONS: What is the isoelectric point of casein?…
A: Since you have posted a question with multiple sub parts, we will provide the solution only to the…
Q: Before we finish this part of the Activity, you’re going to begin summarizing what you’ve learned…
A: Functional Group: A functional group is a reactive portion of a molecule that is responsible for the…
Q: Describe the mechanism of action for the statins.
A: The mevalonate pathway is the metabolic pathway that produces cholesterol and other isoprenoids.…
Q: Cholera is caused by a toxin produced by the bacterium Vibrio cholerae. Ultimately, the toxin leads…
A: G-protein is a GTP binding protein that is found closely associated with the receptor called…
Q: If you wanted to estimate the molecular weight of a newly isolated protein (no known amino acid…
A: If you want to estimate the molecular weight of a newly isolated protein with no known amino acid…
Q: Align the nonapeptide digest fragments in the proper order, from the amino terminus to the carboxyl…
A: Enzymes called as proteolytic enzymes break the peptide bonds within the polypeptide sequence. The…
Q: Which of the following is/are true about Okazaki fragments? 1. Okazaki fragments are found in the…
A: DNA replication is semi-conservative. The double helix of DNA unwinds during DNA replication and…
Q: 3. Tyrosine kinase receptors are pairs of proteins that span the plasma membrane. On the…
A: Enzymes can exhibit cooperativity, which refers to the phenomenon where the binding of a substrate…
Q: OH HO "OH Enzyme A 0= отва OH OH HO OH
A: Glycolysis is a metabolic pathway that converts glucose into pyruvate. The site of this metabolism…
Q: Discuss briefly the role of (a) 10% hydrochloric acid (b) 10% Na2CO3, and (c) anhydrous Na2SO4 in…
A: Alkaloids are a diverse group of naturally occurring compounds that are produced by plants, fungi,…
Q: 1.In this model, the substrate still needs to fit into the enzyme like a key, but instead of simply…
A: Since you have posted multiple questions, we will provide the solutiononly to the first five…
Q: 23. In the structure below, the shaded areas H Ca H Ca H a) are unable to freely rotate around the…
A: Note: Since you have posted multiple questions, we will provide the solution only to the first…
Q: Ingredients: Ham, Swiss cheese, artisan stone-milled rye bread, tomatoes, red onion, lettuce,…
A: INTRODUCTION : Carbohydrates : They are organic compounds also known as long chain sugars which are…
Q: The kinetics of an enzyme were measured as a function of substrate concentration in the presence or…
A: In a enzyme catalyzed reaction, the data can be plotted in a Lineweaver Burk plot. This plot is also…
Q: What are the two monosaccharides that make up this structure? H₂N НО CH3 OH OH H NH₂ N
A: Carbohydrates or carbs are maconutrient consisting of Carbon, hydrogen and oxygen atoms. In nature…
Q: Describe the amino acid illustrated above (at pH = 7.0). A. it is a polar, negatively-charged amino…
A: Every amino acid consists of a amino group and a carboxylic group, and a side chain R group. Amino…
Q: cysteine amino acids together in the formation of a disulfide bond and the biological compound…
A: TLC is a versatile and widely used technique in chemistry and biochemistry for separating and…
Q: When the disaccharide is hydrolyzed, the monosaccharide units that are produced are
A: Carbohydrates are one of the three macronutrients that provide energy to the body, the other two…
Q: 10. Coenzyme used in electron transport. 11. It contains an apoenzyme and a metal ion cofactor. 12.…
A: Enzymes are high molecular-weight proteins that catalyse biochemical reactions. They are also called…
Q: The melting temperature (Tm) of 500 bases long DNA is described by the equation: Tm=81.6+ 0.41(GC %)…
A: The Tm is the temperature at which half of the DNA strands in a double-stranded DNA molecule have…
Q: The parents of a 4-year-old boy noticed that his urine darkens almost to black, after being left in…
A: The black urine disorder is a rare inherited disorder. It is also called phenylketonuria. Conversion…
Q: Discuss the similarities and differences between DNA and RNA. Be specific and use your own words.…
A: DNA stands for deoxy ribonucleic acid where as RNA is ribonucleic acid. Nucleic acids are the…
Q: If the enzyme maltase has a Vo of 0.25 mM per minute when [S] = 0.10 mM, and a Vo of 0.40 mM per…
A: Enzymes kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction…
Q: If the absorbance at540nm of a biuret and protein solution containing 0.25mg/ml of protein .24 the…
A: The biuret assay is a colorimetric method used to quantify the concentration of proteins in a…
Q: Without "unraveling" the following carbohydrates, classify each as a ketose or aldose HO но- HỒ CH…
A: The classification of a carbohydrate as a ketose or aldose depends on the location of the carbonyl…
Q: Explain why people with a hereditary deficiency of carnitine acyltransferase II have muscle…
A: Carnitine acyltransferase II (CAT II) is an enzyme that is involved in the transport of long-chain…
Q: Consider the polysaccharide to the right? a) What is the linkage between the sugar units? b) Is…
A: Disaccharides are carbohydrates made up of two monosaccharide units linked together by a glycosidic…
Q: Create an illustrated step-by-step schematic diagram of the following Analysis of Lipids: a)…
A: Lipids are biomolecules that do not have a fixed chemical structure like carbohydrates or amino…
Q: 1. what cause the reaction of the rancid oil to ph paper to be different from that fresh oil 2.…
A: The biological macromolecules can be classified a nucleic acids, proteins, lipids and carbohydrates.…
Q: Choose in the options what do enzymes do for the activation of energy of the reaction -Ea, given the…
A: Enzymes act as catalysts and catalyse the reactions by increasing the rate of reaction. In collision…
Q: Weak acids in water are _________________________ reactions. Weak bases in water are…
A: Weak acids in water are equilibrium reactions. This means that the weak acid partially dissociates…
Q: The child who fainted was taken by ambulance to the hospital. Objectively: reflexes are absent,…
A: The following steps will explain how accumulation of a specific metabolite leads to appearance of…
Q: The atmospheric ratio of peroxyacetic nitric anhydride (PAN) to PAN + inorganic nitrite varies from…
A: The observation that high values of the PAN/PAN+inorganic nitrite ratio are associated with…
Q: Is the NAM in the explanation supposed to be NAG as shown above in the illustration?
A: Lysozyme is an enzyme that catalyzes hydrolysis of the glycosidic bonds between N-acetylmuramic acid…
Q: tate and define the four levels of protein structure, also include the bonds found at each level?
A: Introduction Proteins are the most abundant macromolecules in our body. Proteins are made up of…
Q: What is an engineered plasmid used for? What are the important features of\ engineered plasmids?
A: Engineered plasmids are powerful tools that allow researchers to manipulate DNA sequences and study…
Q: In ATP synthase, what is the importance of the beta subunit, and how does it interact with the gamma…
A: As per the chemiosmotic model, the proton motive force drives the ATP synthesis. ATP synthase is the…
Q: Acetone, CH3–(C=0) –CH3, and urea, NH2–(C=0)–NH2, have similar chemical structures. (a) Compare the…
A: Acetone, CH3–(C=0) –CH3 - it is an organic solvents which unfolds the protein by interfering the…
Q: Glucose-6 Phosphate Dehydrogenase and TPI Deficiencies 2 H+ Glucose-6-P GSH + GSH Reduced…
A: Introduction G6PD (Glucose-6-Phosphate Dehydrogenase) is an essential enzyme in glucose metabolism,…
Q: The glutamate dehydrogenase (GDH) catalyses the following reaction: H +H3N- C CH₂ CH₂ COO™ acide…
A: In order to calculate the concentration of species in the reaction medium at time =0, we need to…
Q: Find out the classification and examples of antibiotic based on chemical classification
A: The antibiotics are chemical compounds which are used to kill or contain the spread of bacteria.…
Q: A. Draw the structure of the following peptide sequence at pH 2.0: ASP-ALA-THR-LYS-GLY B. What is…
A: Amino acids are basic building blocks of polypeptides, and one or more of these polypeptides they…
Q: Which of the follwing enzymes adds incoming deoxyribonucleotid triphosphates to the 3' OH of the…
A: Deoxyribonucleotides or deoxyribonucleoside triphosphates (dNTPs) are the building blocks of DNA.…
Q: IDENTIFICATION: 1.Complex organic compound w/c has the ability of speeding chemical reactions w/out…
A: Since you have posted multiple questions, we will provide the solution only to the first five…
Q: first pass metabolism
A: The mechanism of first-pass metabolism involves the biotransformation of a drug or substance by…
Q: Sweetzyme IT Extra is a trademarked immobilized enzyme formulation of glucose isomerase sold by the…
A: Vmax = k2[E0] =0.570 s-1 × 2.30×10-5 M = 1.311×10-5 M s-1
Q: Discuss how the cell uses RNA molecules to synthesize proteins.
A: The majority of living things and viruses include the molecule known as ribonucleic acid (RNA). It…
Q: The high phosphoryl transfer potential of 1,3-bisphosphoglycerate is explained by the resonance…
A: Resonance stabilization is a phenomenon in which resonance allows the delocalization of electrons to…
Q: 7) The following are a product of the ester hydrolysis of a lipid which contains three esters.…
A: Simple lipids are esters of fatty acids with glycerol, i.e. it forms ester bond with alcohol,…
Step by step
Solved in 2 steps
- You will be given 1 mL of a 2.0 mg/mL solution of bovine serum albumin (BSA) and 1 mL of a 2.0 mg/mL solution of bovine gamma globulin (BGG). From these solutions, you will need to make 100 μL of each of the following standards using 1x MBP purification buffer as the diluent for preparation of a standard curve: 1000 μg/mL, 750 μg/mL, 500 μg/mL, 250 μg/mL, 125 μg/mL, and 0 μg/mL.This graph represents a standard curve from a BCA assay for protein concentration. If your BCA reaction with your unknown protein has an absorbance of 0.2, approximately what concentration of protein is in your unknown sample? Absorbance (562nm) 0.5 0.45 3 0.35 0.3. 0.25 0.15. 0.1- 0.05 ~0.1 μg/ml ~4.5 μg/ul less that 0.005 | 0.2 μg/μ. [Protein (BSA)) (pg/ml)A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a Bradford assay. The increase in absorbance at 595 nm relative to the concentration of protein was plotted as shown below. Save 1.0- 0.8- 0.6- 0.4- 0.2- 0.04 0.0 0.5 1.0 2.0 2.5 1.5 BSA concentration (mg/mL) a) Using the graph, calculate the original concentration of protein present in a mixture of haemoglobin and methylene blue (diluted 1:50), which gave an absorbance of 0.20 at a wavelength of 595 nm using the Bradford assay. Show all workings. Absorbance (O.D.)
- You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.During a mass spectrometry experiment, the spectrum of vascular endothelial growth factor A (VEGF-A) was acquired. The absolute error of VEGF-A, having a mass of 1024.5210 Da was 0.06 Da. Calculate the relative error (ppm).You put 50 uL of 0.1 mg of protein into a 1 cm pathlength cuvette containing 3 mL of LDH reaction mixture and measure a slope of 0.125 abs/min in an LDH assay. What is the reaction rate in uM/min? Show your work.
- A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: glutamate dehydrogenase, 290 kDa with elution volume 20.8 mL; serum albumin, 37 kDa with elution volume 42.5 mL Use these values to determine the equation of the straight line graph that relates log molar mass to elution volume. The elution volume of an unknown protein was 32.2 mL. Use the equation just determined above to estimate the molar mass of the protein in kDa units. Don't round your answer until the end to ensure your answer is within the accepted range. Round your answer to the nearest whole number (e.g. If you calculated 27.52, input your answer as 28).Which of the following is incorrect about blood glucose test interpretation A) fasting plasma glucose 6.1-6.8 defined as having IGT and should probably be screened again after a year or so. B) 2 results>7mmmol/l defined as having diabetes C) fasting plasma glucose >6mmol, recall for further test. D) fasting or random plasma glucose, on one reading of <11.1mmol/l is diagnostic of diabetesYou have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose. Please answer with explanation ASAP. I will really upvote. Thanks
- From this standard curve and chart below, does the separation of molecules in the mixture appear successful from the gel filtration? Is there a clearlydefined separation between molecules? Explain your conclusions. Parameters required for calculation of coefficient (Kd) for unknown protein Volume eluted (mL) Which variable does this volume represent in the equation for Kd? Fraction with maximal DNP-Aspartate detected 36 Vt Fraction with maximal Protein detected 24 Ve Fraction with maximal Blue dextran detected 6 VoThis is a molarity problem that I cannot figure out. If you could please help me understand the steps to solving it that would be greatly appreciated! Question: We need to prepare a stock solution of medium for your culture cells, which usually includes liquid salt solution and bovine serum. Our liquid salt solution is supplied in a 50X concentration, and we need to dilute it to 1X for use. We also need to add 75% fetal bovine serum for a final concentration of 15%. How would we make up 0.80 liters of this culture media using water as our solvent?Suppose that you are tasked to determine the protein concentration of an unknown protein solution via Bradford assay. You prepared six solutions of bovine serum albumin (BSA) with different concentrations. The initial concentration of the BSA stock solution is 7.50 mg/mL. Approximately 200 µL of Bradford Reagent was added to each of these solutions and the absorbance at 595 nm was taken after 5 minutes. See the table below for data on the standard solutions. Standard # A595 BSA conc (mg/mL) 0.000 0.158 2 1.125 0.291 2.250 0.372 4 3.375 0.503 5 4.500 6 5.625 0.675 Determine the protein concentration, in mg/mL, of the unknown solution if its absorbance at 595 nm was 0.248. Note: Final answer format must be x.xx (two decimal places). Round off only in the final answer. Do not round off in the middle of calculation.