Compare the experimentally defined locations of the amino acids from the SCAM data with the positions predicted in the topological model. Comment on whether these experimental data are consistent with the topology model; do the data support this model? If not, how do they differ?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question

Compare the experimentally defined locations of the amino acids from the SCAM data with the positions predicted in the topological model.

Comment on whether these experimental data are consistent with the topology model; do the data support this model? If not, how do they differ?

kDa
55 Cysless
35
20
10
F16C
E39C
L860
G125C
+
+
+U
M18C
V51C
Y96C
F139C
M36C
K72C
---+ MTSES
+ - NEM
+ + + MPEG5K
|D106C
+P
←U
R143C
+P
←U
+ MTSES
- NEM
+ MPEG5K
+P
+U
+ MTSES
- NEM
+++ MPEG5K
+P
←U
+ MTSES
NEM
+++ MPEG5K
Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell
lysates from cells harbouring plasmids expressing cysteine-free
('Cysless') or single cysteine variants of alaE with and without treatment
with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein
are indicated with arrows.
Transcribed Image Text:kDa 55 Cysless 35 20 10 F16C E39C L860 G125C + + +U M18C V51C Y96C F139C M36C K72C ---+ MTSES + - NEM + + + MPEG5K |D106C +P ←U R143C +P ←U + MTSES - NEM + MPEG5K +P +U + MTSES - NEM +++ MPEG5K +P ←U + MTSES NEM +++ MPEG5K Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell lysates from cells harbouring plasmids expressing cysteine-free ('Cysless') or single cysteine variants of alaE with and without treatment with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein are indicated with arrows.
CO
w
Transcribed Image Text:CO w
Expert Solution
steps

Step by step

Solved in 3 steps with 1 images

Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON