Can simply observing the colonies formed on agar plates and the results of a few biochemical tests confirm how many different types of bacteria are present i
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Can simply observing the colonies formed on agar plates and the results of a few biochemical tests confirm how many different types of bacteria are present in coconut water samples?
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- Can simply observing the colonies formed on agarplates and the results of a few biochemical testsconfirm how many different types of bacteria arepresent in coconut water samples? Explain.Can simply observing the colonies formed on agar plates and the results of a few biochemical tests (catalase, gram stain and oxidative/fermentation test) confirm how many different types of bacteria are present in coconut water samples?Compare and contrast the growth of bacteria in different physical types of media (broths, slants, and agar plates). What might be the advantages and disadvantages of using each type?
- A soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.Are the large numbers of microorganisms found in the mouth cause for concern? Explain. How can you determine whether a culture or subculture is pure? What kinds of clinical speciments may yield a mixed flora in bacterial cultures? Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?
- You are given a 1 gram soil sample of unknown bacterial load. After doing 10-fold serial dilutions of the soil in sterile water, 100 uL volumes are taken from each dilution for preparation of pour plates. Following incubation, each half of the 10-8 plate has 46 colonies.a) What was the dilution factor?b) How many bacteria were present in the soil?2. Staphylococcus aureus divides every 20 minutes. A culture begins with 10 bacterial cells.a) After 5 hours, how many generations have occurredb) After 5 hours, how many bacteria are present?3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldHow is agar in the petri dish prepared to grow the bacterial specimen? explain in your own words.
- If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many fewer bacteria the broth used for the plate picture here), would you expect to see single, isolated colored in quadrant 4 or quadrant3? Explain your answer.what is the physical characteristics of this streak plate? Look at a single colony on a streak plate and look for special physical characteristics such as: motility, possible presence of endospores and/or capsule, culture color?Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?