Q: A culture of E. coli has a concentration of 5 x 108 cells/mL. How many times do you have to dilute…
A: Given: 1. Number of colonies: 2502. No. of bacteria in culture: 5 X 108 3. Volume plated: 0.1 ml 4.…
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Q: Explain why a green metallic sheen is formed when E. coli is grown on Eosin Methylene Blue Agar.
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A: The answer for the present MCQ has been explained with definition and in the following step.
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Q: How would you inoculate a plate to get a 1:10 dilution? A 1:100 dilution?
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a single agar plate can be used to quantitatively measure the MIC value of multiple antibiotic aganist a certain bacteria by?
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- For the pour plate method, state which plate will be chosen for colony isolation and why? From the chosen plate, indicate (circle) the colonies for both bacterial species that will be selected and transferred to new nutrient agars as a pure culture.A culture of E. coli has a concentration of 5 x 108cells/mL. How many times do you have todilute the culture so that when you spread 0.1mL on an agar plate you will have 250 colonies?Hint: you need to find dtotal and then convert it into a DF.You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate method what other methods could you use to separate the bacteria? Please state what methods you would use, the result you would be looking for and interpret the result.
- Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Formula: C1V1 = C2V2 *Concentration *Volume Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many fewer bacteria the broth used for the plate picture here), would you expect to see single, isolated colored in quadrant 4 or quadrant3? Explain your answer.Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000
- After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?Mannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?If an organism that showed no growth in the MIC broth test at a particular dose, then grows in an MBC test when plated to media, the concentration of the drug was bacteriostatic. O 1) True O 2) False
- a.Describe what makes thioglycollate medium suitable for culturing anaerobes. What would the growth patterns of Clostridium sporogenes and Micrococcus luteus be in this medium? b. In the Kligler test, why do we inoculate the surface of the agar slope and then stab into the butt of the slope? What does a pink coloured colony indicate when using MAC (MacConkey Agar)?What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of an E-test strip.Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?