) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula.
) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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1) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula.
2)Calculate the required gram of EDTA to prepare O.5M, 100 ml EDTA (EDTA stock) mol and Molarity formula.
3.) Calculate the required volume of Tris Stock to prepare buffer A which contains 25 mM Tris-Hcl with final volume is 100 ml by using M1V1 =M2V2 formula
4) Calculate the required volume of EDTA stock to prepare buffer A which contains 10 mM EDTA with final volume is 100 ml by using M1V1 =M2V2 formula
5)Do the same for Buffer B calculations
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