1. In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored? 2. Explain the Donnan Membrane Phenomenon. Why is it important for the homeostasis of the cell?
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1. In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
2. Explain the Donnan Membrane Phenomenon. Why is it important for the homeostasis of the cell?
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- 6. Imagine the experiment below where you placed a dialysis tube (cell) containing Solution A Dialysis Tube Solution B 10% NaCl (solution B) into a beaker of distilled water (solution A). Using what you know about diffusion/osmosis, indicate if the water is going to move into or out of the dialysis 'cell'.6. A carrot, when dried out, becomes soft. There is a way to make it firm and crisp. Explain how and why, using the following guidelines. a) Draw and label a representative cell of the carrot, in its dried state. Be precise in your drawing. b)Describe the type of extracellular solution that is appropriate to add to make the desired change (name and define what type of solution, the net movements of solvent and solutes, etc.). c)Draw a representative cell of the carrot, in its possible state after the change. Represent the movement of water using an arrow to indicate what has happened.7. Imagine that the red blood cells shown below are suspended in liquids of 3 different concentrations. a. Identify the solution in each box as either hypertonic, hypotonic, or isotonic. b. Describe the reasons for your choice of tonicity. Solution 1 2 3 Red Blood Cells Top-Down View Side View Tonicity of Solution? Reasoning?
- 5. Intravenous solutions must be prepared, to that they are isotonic to red blood cells. A0.9% salt solution is isotonic to red blood cells. Keeping this in mind, explain whatwould happen to a red blood cell placed into a solution of 99.3% water and 0.7% salt.Note to obtain full marks you must care both the intercellular environment to theextracellular environment (solute vs solvent).8.1f you collect a swab of cells from the inside of your mouth and perform the Gram stain technique, what would be the result? Would your epithelial cells be Gram positive or Gram negative? Explain the result based upon the cell structure8. Phosphate buffered saline (PBS) is often used as an isotonic solution to keep cells happy and alive. Though recipes vary, PBS typically contains 157 mM Na* and 4.5 mM K+, yet inside of the cell that ratio is switched (there is approximately 150 mM K* and only about 10 mM Nat). Why is PBS still considered isotonic? That is, why does putting cells into PBS not create a lot of osmotic pressure?
- 8.Patients who enter the emergency room dehydrated are usually given sterile saline solution intravenously. The solution administered is isotonic to prevent cell damage. What would happen if the solution was hypotonic? Group of answer choices A. Hypotonic solutions have a higher osmotic pressure than that within cells so hemolysis can occur B. Hypotonic solutions have a lower osmotic pressure than that within cells so crenation can occur can occur. C. Hypotonic solutions have a lower osmotic pressure than that within cells so hemolysis can occur. D. Hypotonic solutions have the same osmotic pressure as that within cells so nothing will happen. 9.The solubility of CaCl2 in water is135g/100 ml. What would be the best method for preparing a 50.0 mL of supersaturated CaCl2 solution? Group of answer choices A. add 72 g of CaCl2 to 50.0 mL of H2O at 100 °C while stirring at room temperature B. add 67 g of CaCl2 to 50.0 mL of H2O at 100 °C while stirring at room temperature…A doctor injections a patient with what the doctor thinks is isotonic saline solution. The patient dies,and autopsy reveals that many red blood cells are swollen or completely destroyed. 1. Do you think the solution the doctor injected was really isotonic.2. Assuming that the doctor did not inject an isotonic solution what was the tonicity of the solution he injected 3. Explain what caused the patients red blood cells to swell. Incorporate into your answer three or more following terms . Hypotonic ,isotonic,high concentration, lower concentration, lower concentration,tonicity,extracellular,intracellular 5. Before refrigeration, it was a common practice to salt meat in order to prevent bacterial growth and decay. Propose an explanation for why this process prevented bacterial growth. Diffusion Write a summary of your results and answer the questions. SUMMARY 1. Based on molecular weight, which dye would you predict to diffuse the furthest? Justify your choice. 2. What is the relationship between diffusion rate and temperature? Justify your statement with your results. What is your explanation for this relationship?
- 3 cell membrane samples: A, B, and C were fluorescently labeled and subsequently photobleached. The recovery of fluorescence is given on the FRAP curve: (see image attached) Rank the 3 cell membranes in terms of DECREASING fluidity and explain your ranking based on their FRAP curves, most probable appearance, and composition of the membranes. Draw the expected image of the surface of the 3 membranes BEFORE photobleaching and AFTER fluorescence recovery as viewed under a fluorescence microscope.Red blood cells are dropped in a solution of unknown concentration. Looking through the test tube, the image behind is cloudy. Upon microscopic examination we see that the cells seem to have shriveled up. What does this tell us about the solution? O It is isotonic O It is hypotonic O It is hypertonic10. What if you placed the same cells into pure, distilled water (0% saline solution)? (Circle one answer.) The cells were immersed in a hypotonic/isotonic/hypertonic solution. 11. What if you put the same cells in a 2% saline solution? (Circle one answer.) The cells were immersed in a hypotonic/isotonic/hypertonic solution. 12. Based on your understanding of osmosis, why is it very important for intravenous fluids given in a hospital to have the same osmotic concentration as your own cells and body fluids?