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- Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with mold(1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specific
- Considering microbial identification methods, to which category would the following method belong based on what it measures? Examination of the shape and height of bacterial colonies growing on a plate O Phenotypic Genotypic OSerological Biochemical MacBook AirDescribe how you would execute this first stage from the point you are handed the nutrient broth tube containing the mixed culture, through to the appearance of two colony types on a nutrient agar plate. Assume you have all the necessary equipment and materials at your disposal. Be concise, but thorough; limit yourself to a short paragraph (1/4-1/2 page at most) – include the method and techniques; what you expect to see, and how you would avoid contamination during the process.What are the primary uses of the following preparations of culture media? Agar regular slant Agar special slant Agar deep Broth Agar plate
- Provide three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory.What is the major difference between an enrichment culture and a selective culture? Why are microbial doubling times in nature typically longer than those obtained in the lab? Briefly describe the following mechanisms of measuring bacterial growth: Direct microscopic cell count Plate count Most probable numberWhy might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Discarding dirty, used micropipette tips is one way to practice sterile technique. What are some other ways to practice sterile techique in lab this week? Check all that apply. Wear gloves while working with the EcoPlate, micropipette, and soil serial dilutions. Leave your sterile tubes uncapped and open, for airborne bacteria to enter. Keep food and drink off your lab bench. Remove your gloves before handling your computer, phone, etc. Use provided ethanol solution to clean your lab bench. Touch the soil or soil solutions with your bare hands or fingers.A swab for culture is received from a doctor's office. The staff forgot to mention thesource from where the swab was taken and the climc was closed for next few days.Whatdifferent selective/differential media may be helpful to you to identify the organism.Explain very briefly your rationale for using the particular culture media.