Which of the following is true Select one: O a. Inoculating loop and needle are culture equipment used for transferring cultures O b. Dropping Pipettes are culture equipment used for transferring cultures Oc. Test tubes are transfer equipment used for transferring cultures O d. Inoculating loop and needle are transfer equipment used for transferring cultures.

Which of the following is true Select one: O a. Inoculating loop and needle are culture equipment used for transferring cultures O b. Dropping Pipettes are culture equipment used for transferring cultures Oc. Test tubes are transfer equipment used for transferring cultures O d. Inoculating loop and needle are transfer equipment used for transferring cultures.

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter26: Pediatrics

Section: Chapter Questions

Problem 9CR

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An inoculating loop must be flamed to uniform orange-hot from base to tip A. after culture is transferred to the wire loop but before transfer to new media B. before culture is transferred to the wire loop and after culture is transferred from the wire loop to new media C. after culture is transferred to the wire loop and before culture is transferred from the wire loop to new media D. before culture is transferred to the wire loop
In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar plates b. Micropipettor c. Inoculating needle d. Alcohol lamp or Bunsen burner e. Test tubes or microcentrifuge tubes
Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus pyogenes from a patient’s throat swabb. isolating a pathogen from a patient’s clean-catch urine samplec. isolating enteric bacteria such as Escherichia coli from a sample oforganically grown spinach d. maintaining a patient’s nasal swab specimen for further analysis andidentifi cation of possible respiratory syncytial virus (RSV) infection
What type of medium is best used to maintain a preserve specimen before clinical analysis? A. Enriched b. Selective c. Differential d. Reducing e. Nutrient
Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
Refer to the following diagram: 100 ul 100 uL 100 pl 100 µL 100 pl 100 pL 100 pl Volume to transfer A B C D E F G 900 uL in each tube 10 102 10 10 10 10 10 Dilutions E.CoN culture Volume of the diluted sample plated 100 ul 100 μL 100 pL 100 ul Volume of the 10 ml 10 ml original sample plated 10 ml 10 ml Plate D Plate E Plate F Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.
You are rotating sterile supplies based on the first in, first out (FIFO) principle. What would you look at? O Sterilization method Expiration date Manufacturing date Packaging material
Below is a diagram of the serial dilution of a culture with 1 x 10º cells. Fill out the missing information. 250 Culture Diluent 1 x 10° cells/mL 9 mL 9 mL 9.9 mL 9.9 mL 99.9 mL Volume to add to diluent Final Dilution level Theoretical count after plating 100 uL
1 ml of a patient sample was added to 999 ml of media. From this 0.1 ml was transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution factor here. What's the number of bacteria per ml of the original patient sample. a. The dilution factor is 1000. The patient sample contains 600 cells/ml b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml c. The dilution factor is 100. The patient sample contains 600,000 cells/ml d. The dilution factor is 10. The patient sample contains 6000 cells/ml e. It cant be determined its too many cells
While conducting the Hydrogen Sulfide Test (SIM Agar) you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Please explain how the color change is to occur. Think critically.
Which of the following is not an example of aseptic techniques? O Working in the presence of a flame. O Flaming the mouth of the test tube after use. Heat fixing the specimen. Flaming the loop before and after transfer. O Keeping Petri dishes closed when not in use,
1. A bacterial culture was grown for 12 hours. At 4-hour interval, the culture was sampled to determine the population ofthe culture, by transferring 25 mlof the suspension to 225 ml 0.90% NaCl. Three consecutive dilutions were further made by using 1 mlaliquot in 9 mlof 0.90% NaCl. One ml from each dilution was plated in each of duplicate plates. The following table shows the results of the plating method. EDI Sampling COUNTS 1st dilution 54; 61 TNTC 2nd dilution 3; 7 TNCT TNTC 3rd dilution 0; 0 242; 233 TNTC 4th dilution 0, 0 28: 37 249;246 1st 2nd 3rd TNTC * TNTC = Too numerous to count i. Determine the bacterial count (CFU/ml) every 4 hours of incubation for 12 hours. Show all computations. a. 1s* Sampling b. 2d Sampling c. 3* Sampling