Ap Normal No Spacing Head 目三。、田 Paragraph Styles 1. You heve a circuler plasmid (pUC57), which consists of 2710 bp. What would the number and length of the fragments be if you cut the plasmid with the following restriction enzymes or a combination of enzymes2 Show all calculations. a. Edml & Gsul b. Ndel. Sanl & BseXI c. Scal, Sspl & Ebel !!! lili
Ap Normal No Spacing Head 目三。、田 Paragraph Styles 1. You heve a circuler plasmid (pUC57), which consists of 2710 bp. What would the number and length of the fragments be if you cut the plasmid with the following restriction enzymes or a combination of enzymes2 Show all calculations. a. Edml & Gsul b. Ndel. Sanl & BseXI c. Scal, Sspl & Ebel !!! lili
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Transcribed Image Text:EAL T
Normal
No Spacing
Head
Paragraph
Styles
1. You have a circuler plasmid (PUC57), which consists of 2710 bp. What would the
number and length of the fragments be if you cut the plasmid with the following
restriction enzymes or a combination of enzymes? Show all calculations.
a. Pdml & Gsul
b. Nidel, Sapl & BseYL
C.
c. Scal, Sspl & Ebel
2 You want to clone a specific PCR amplicon. You have determined that the amplicon
you want to clone has enzyme restriction sites for BamHl and EcoRI. After investigation
you see that the pUC57 plasmid also has these enzyme restriction sites in its multiple
cloning site (MCS on map above, start at 396 bp and ends at 476 bp). After enzyme
digestion your amplicon that you wish to insert is 914 bp long.
a. What will be the length of the recombinant plasmid after you have cloned your
amplicon? Show your calculation(s).
b in the amplicon insert you have an enzyme restriction site for Ndel at 864 bp.
If you digest the recombinant plasmid with this enzyme what length will the
fragments be? Show your calculation.
y istigate
DiF
PLDECE
52
![Ao三
Normal
No Spacing
Hea
A-
= = =国位。|鱼
三、 、■
Styles
Paragraph
Section B: Circular DNA
[12]
Eco01091 2698 Pfol 46 BstAPI 179
Aatil 2641
Sspl 2525
Ndel 183
Ehel 235
Pdml 2318
396
MCS
lacz
lac.
146
2510
Bcgl 2239
Scal 2201
236
476
493
PUC57
2710 bp
BsaX 683
Sapl 707
2/6
Afll, Psel 830
Gsul 1808,
Cfr101 1803.
Eco311 1790.
Eam11051 1718
1650
1400
rep (PMB1)
BseYl 1134
Cail 1241
CAFT TR
Lad l
The d Mat The M
1.
You have a circular plasmid (pUC57) which consisrs of 2710 bo. What would the
number and length of the fragments be if you cut the plasmid wih the following
essibility: Investigate
52
OO C a
bla (Ap)
EGO](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F701effb4-d604-4206-a385-859591afa082%2Fd5d97b5f-4139-401a-ba67-2c6ab11dd52a%2F0gdmt1a_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Ao三
Normal
No Spacing
Hea
A-
= = =国位。|鱼
三、 、■
Styles
Paragraph
Section B: Circular DNA
[12]
Eco01091 2698 Pfol 46 BstAPI 179
Aatil 2641
Sspl 2525
Ndel 183
Ehel 235
Pdml 2318
396
MCS
lacz
lac.
146
2510
Bcgl 2239
Scal 2201
236
476
493
PUC57
2710 bp
BsaX 683
Sapl 707
2/6
Afll, Psel 830
Gsul 1808,
Cfr101 1803.
Eco311 1790.
Eam11051 1718
1650
1400
rep (PMB1)
BseYl 1134
Cail 1241
CAFT TR
Lad l
The d Mat The M
1.
You have a circular plasmid (pUC57) which consisrs of 2710 bo. What would the
number and length of the fragments be if you cut the plasmid wih the following
essibility: Investigate
52
OO C a
bla (Ap)
EGO
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