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What does DNA polymerase need in order to make contact with a replication origin?
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- How many replication forks are formed at the origin of replication?In DNA replication, the role of topoisomerase is to Question 11 options: a) "unzip" the double stranded DNA in front of DNA polymerase. b) maintain the single stranded DNA. c) supercoil the DNA after the replication fork has passed. d) relieve supercoil tension in the DNA in front of the replication fork.Why is the replication of DNA referred to as a semiconservative process? What is the experimental evidence for the semiconservative nature of the process? What experimental results would you expect if replication of DNA were a conservative process?
- In DNA replication, there are leading and lagging strands because A) DNA replication is conservative and a completely new DNA molecule must be made. B) DNA replication is semiconservative and each strand is copied simultaneously in opposite directions. C) the strands of DNA are parallel and are copied in the same direction simultaneously. D) one strand of DNA is copied faster than the other.What are the chemical bonds of the DNA molecule that are broken for the replication process to occur?Regarding the process of DNA replication, it is correct to state that: a) Nucleosomes are maintained during the DNA polymerase-mediated replication process b) The phosphodiester bonds that join the nitrogenous bases maintain the integrity of the DNA strands c) DNA polymerase requires a previously annealed deoxynucleotide to add the next monomer being synthesized. d) The action of the helicase decreases the twist caused by the movement of the replication complex along the stretch of DNA e)
- What is the difference between the leading strand and the lagging strand in DNA replication? There are different DNA polymerases involved in elongation of the leading strand and the lagging strand. The leading strand is synthesized continuously in the 5' → 3' direction, while the lagging strand is synthesized discontinuously in the 5' → 3' direction. The leading strand requires an RNA primer, whereas the lagging strand does not. The leading strand is synthesized in the 3' → 5' direction in a discontinuous fashion, while the lagging strand is synthesized in the 5' → 3' direction in a continuous fashion.What is a replication fork? Why is it important in replication?Which of the following statements is false about DNA replication? A) Each lagging strand of DNA is started by an RNA primer. B) DNA polymerase joins nucleotides in the 5' to 3' direction only. C) The leading strand of DNA is made continuously. D) More than one replication fork is present. E) A replication bubble opens in one direction only.
- What is bidirectional replication?2) Replicating structures in DNA can be observed in the electron microscope. Regions being replicated appear as bubbles. a) How many replication forks are present? b) Assuming bidirectional replication, how many origins of replication are active in this DNA molecule? c) Assuming that all replication forks move at the same speed, which origin of replication was activated first (left, middle or right)? Why?What mechanism was originally proposed as one of the three models for DNA replication? What is the name given to the short stretches of DNA formed on the lagging strand? How can these short fragments be initiated?