Objective lens A has a limit of resolution equal to 0.2 mm while objective lens B has 30 μm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing
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- Objective lens X has a limit of resolution equal to 0.2 mm while objective lens Y has 30 µm. a. Which among the two can magnify a mosquito wing in finer detail? b. Which among the two would show a greater area of the mosquito wing?What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.Use the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?
- The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?What is the rule for determining total magnification? A. Ocular lens magnification + objective lens magnification = total magnification B. Ocular lens magnification - objective lens magnification = total magnification C. Ocular lens magnification x objective lens magnification = total magnificationExplain how total magnification is calculated. Select an answer. a. It is the magnification of the objective lens multiplied by the magnification of the condenser lens b. It is the magnification of the objective lens multiplied by the magnification of the ocular lens c. It is the magnification of the objective lens used d. It is the magnification of the ocular lens used
- Total Magnification is obtained by Select one: A. Magnifying power of both the objective lens and eyepiece B. Magnifying power of eyepiece C. Magnifying power of condenser lens D. Magnifying power of the objective lensHow many coccus cells (0.0005 mm in diameter) could fit across the entire FOV of the 100x objective lens (assuming the DFOV of the 100x lens is 180 μm?Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
- How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!the figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's Law