An electrophoretic separation of lactate dehydrogenase isoenzyme that demonstrates elevation in LD-1 greater than LD-2 could be indicative of: Please select the single best answer A normal LD isoenzyme pattern Hemolysis Pancreatitis Hepatic injury
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An electrophoretic separation of lactate dehydrogenase isoenzyme that demonstrates elevation in LD-1 greater than LD-2 could be indicative of:
Please select the single best answer
| A normal LD isoenzyme pattern | |
| Hemolysis | |
| Pancreatitis | |
| Hepatic injury |
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- You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below. Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase 45% Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate Glutamic dehydrogenase? © 20% O 30% 45% 65% 80%Select the enzymes that play a role in producing monosaccharides absorbed by SGLT1. Group of answer choices Pancreatic alpha-amylase Sucrase Maltase Isomaltase Lactase alpha-amylase beta-amylase Glucoamylase Pullulanase Endoglucanase Exoglucanase Cellobiase All of the aboveCori’s disease, is an autosomal recessive metabolic disorder that is characterized by defects in the glycogen debranching enzyme amylo-1,6-glucosidase,4-α-glucanotransferase (AGL). It primarily effects the Liver, heart and skeletal muscle. Symptoms can include hyperlipidemia, ketoacidosis, and hypoglycemia. The possible dietary recommendations are as follows:1. A high protein diet to facilitate gluconeogenesis2. A ketogenic diet to provide an alternate source of energy3. Small frequent meals to prevent hypoglycemia4. A high fat dietWhich dietary recommendations can be used to treat this disorder? a) 2 and 4 b) 1 and 2 c) 2 and 3 d) 1 and 3 e) None of the above recommendations would be effective f) 3 and 4 g) 1 and 4
- Lactose intolerance is caused by reduced production of the enzyme lactase. A variety of digestive system problems, such as celiac disease and Crohn’s disease) can also lower the production of lactase. If an individual produces low levels of the enzyme lactase, explain why they might be able to tolerate eating dairy products that contain lower levels of lactose (e.g., hard cheeses, feta cheese, and cottage cheese), but will not be able to tolerate foods with high levels of lactose (e.g., milk).What is the role of pyridoxal phosphate in transaminase determination? What is the clinical significance of elevated AST? ANSWER IN 3-5 SENTENCESCreate or design a concept map explaining in one page using figures, illustrations, and a few descriptions or explanations of the assigned glycogen storage and lipid diseases in your group. The content of your concept map should include the biochemical and molecular basis of the type 0 glycogen storage disease with clinical presentation and therapeutic options
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