After taking an aliquot of your cells and diluting them with Trypan Blue in a 1:1 dilution, you load thecell/Trypan mixture onto a hemocytometer to count the cells.You obtain the following counts:Quadrant Transparent cells Blue cells1234LO6557168732413674) If your original cell culture (the one you took an aliquot from to count) has 13 mL of cell suspension, what isthe total number of cells you have in culture?Provide your answer as the full number2

Hemocytometer is an instrument which is used to count the number of cells present in a sample under…

After taking an aliquot of your cells and diluting them with Trypan Blue in a 1:1 dilution, you load the cell/Trypan mixture onto a hemocytometer to count the cells. You obtain the following counts: Quadrant Transparent cells Blue cells 2 1 2 3 4 65 71 68 73 4 1 3 5 67 4) If your original cell culture (the one you took an aliquot from to count) has 13 mL of cell suspension, what is the total number of cells you have in culture? Provide your answer as the full number 2

After taking an aliquot of your cells and diluting them with Trypan Blue in a 1:1 dilution, you load the cell/Trypan mixture onto a hemocytometer to count the cells. You obtain the following counts: Quadrant Transparent cells Blue cells 2 1 2 3 4 65 71 68 73 4 1 3 5 67 4) If your original cell culture (the one you took an aliquot from to count) has 13 mL of cell suspension, what is the total number of cells you have in culture? Provide your answer as the full number 2

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter4: Basic Immunology And Immunohematology

Section4.3: Tests For Rheumatoid Factors

Problem 2.1CS

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Please note that these counts have all been done with Trypan Blue. Therefore, all cultures have been diluted two-fold (equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.1These numbers represent the total number of cells counted in 5 large squares from three separate counts.
You are determining the concentration of your culture, and have counted the following on your hemocytometer as shown in Figure below: (note, blue cells have taken up Trypan blue & thus are dead. Red x's are cells that should not be counted because touching one of 2 sides of square). If your cells were diluted 1:2 before counting, what is the concentration of cells in your original culture?
Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of amL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further dilutedby successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gramsample of hamburger?
Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability (b) for this sample. (assume we started with 100 microliters of cells and added 100 microliters of trypan viability blue). Hemacytometer Grid 123 5 6 789 4 Enlarged view O O O 8
You counted 4, 6, 12, 3 cells in each of the 4.outed squares of a hemacytomeyer. What are the cells per milliliter in that culture? If you resuspended your cell pellet 2.5 mL, what is the total cell count? How many uL do you need to add to a new culture if you want 4250 cells?
You want to determine the amount of cells in a culture. You dilute the suspension to 10^-4 and plate 100ul onto an agar plate. After overnight incubation there are 30 colonies on your plate. How many cells are in your original suspension (assume your culture is 1 Liter)?
A 5mL sample diluted 1:1 with trypan blue is counted using a hemocytometer. A total of 325 viable cells and 52 dead cells were counted in the four corner square and the big central square of the meter. What is the total number of dead cells per mL of the sample?
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You are added 60mL of soil slurry to 540mL of sterile water. You can transferred 1000uL of that dilution to 9 mL of water. You then prepared a 10-7 dilution from that tube. You finally transfer 1 uL of that to a TSA plate. After 48 hours, you counted 32 colonies on quarter of the plate. (A) How many cells/mL were present in the original soil sample? (B) How many colonies would be present on the plate if you would have plated 1mL of the last sample? (C) How many milliliters would you need to prepare a 10-4 from 100uL of sample?
Jon performed a pour plate technique to determine the concentration of viable cells in her mother culture for nata de coco production. Prior to all the samples, he poured an extra Petri dish without any sample with the molten agar. What is the purpose of doing this?
A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells were counted in 25 small squares (each of volume 2.5 x 10-7 cm3). Viable counts were carried out on the same culture using both the pour plate method (incorporating 1 mL samples of a range of dilutions into plate count agar plates) and the Miles & Misra spread plate method, where 0.02 mL samples were spread on sectors of a plate count agar plate. For the pour plate count the average of triplicate samples was 178 colonies per plate of the 10-4dilution. For the Miles and Misra count the average of triplicate samples was 21.3 colonies per sector of the 10-4 dilution. Calculate the total count and the viable pour plate and spread plate counts and suggest possible reasons for the difference between the three different counts.
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