ACTIVITY 7.1.1 Practice drawing the structure of all 5 bases including the numbering of the atoms of the rings. Which bases are found in DNA? In RNA?
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- ACTIVITY 7.2.3 Ja. Write the structural formula of GAC, a portion of DNA. Write the complementary strand adjacent to it so that the complementary bases are side by side. Connect the appropriate base pairs. b. Sticking to the convention of writing the nucleotide sequence in the 5'-3' direction, what is the nucleotide sequence of the DNA strand complementary to ATGCACCATGCT?ACTIVITY 7.3.2 1. Name differences between replication and transcription. 2. Write the sequence of the RNA transcribed from the following DNA: 5' GATCAATGCTAG 3' 3' CTAGTTACGATC 5Question Guide: 1. Name and write the structures of the purine and pyrimidine bases present in DNA. Purine Рyrimidine Structures: Purine Pyrimidine
- ACTIVITY 7.3.2 1. Name differences between replication and transcription. 2. Write the sequence of the RNA transcribed from the following DNA: 5' GATCAATGCTAG 3' 3' CTAGTTACGATC 5' 152 Copyright 2019. All Rights Reserved.ACTIVITY 7.3.3 1. Given the MRNA: 5'AUGCAUGACGAUCUCGUCGCG....3' a. Use the genetic code to predict the amino acid sequence encoded by the mRNA. b. Naturally, the information in this MRNA came from DNA. Give the sequence of the sense strand of the DNA. 2. Referring to the mRNA above, a tRNA must interact with the MRNA codon CUG which codes for Leu. What will be the anticodon in the TRNA-Leu?ACTIVITY 7.3.3 1. Given the mRNA: 5'AUGCAUGACGAUCUCGUCGCG...3' a. Use the genetic code to predict the amino acid sequence encoded by the mRNA. b. Naturally, the information in this mRNA came from DNA. Give the sequence of the sense strand of the DNA. 2. Referring to the mRNA above, a tRNA must interact with the MRNA codon CUG which codes for Leu. What will be the anticodon in the tRNA-Leu? 153 Copyright 2019. All Rights Reserved.
- TRUE OR FALSE 1. Nitrogenous bases cannot undergo electrophilic aromatic substitution.2. Cytosine forms three hydrogen bonds with guanine while Adenine forms two hydrogen bonds with Thymine in themessenger RNA.3. The glycosidic bond in nucleotides is stable in dilute ammonium hydroxide solution and dilute Sodium bicarbonatesolution.4. In gel electrophoresis, a DNA is expected to migrate towards the cathode.5. Tautomerism is a phenomenon observed in both purine and pyrimidine bases.TRUE OR FALSE] 11. The structure of the DNA, being super coiled, promotes increased viscosity of the molecule.12. The stacking position of the nitrogenous bases is caused by intramolecular Van der Waals Interaction.13. The histone complex is a quaternary protein structure.14. The difference between ribose and deoxyribose is in the presence of oxygen in carbon number 3 of the sugar moiety.15. The sedimentation coefficient of the nucleic acid is higher compared to ribosomes.Activity 9a -DNA and RNA Structure 1. Fill in the table with the characteristics of DNA and RNA. Function Which four bases will you find? double stranded or single stranded Sugar found in the molecule DNA 2. What are the three parts of a nucleotide? RNA
- 9. (a) Q1 a. Justify why the base thyamine is prefered over guanine in DNA while the reverse is preferred in RNA. Provide the chemistry involved to support your answer.Part II: Information Transfer Background Information - Key Points The background information provided for this lab has given you a general overview of some of 24 the key terms and definitions necessary to understand the transfer of information from gene to protein. The information included below will help you work through the specific problems included in your Tutorial 4 Assignment. When working on the problems remember the base mu to pairing rules (Table 3). Table 3: Rules for nucleotide base pairing. cytosine (C) - guanine (G) adenine (A)- thymine (T) DNA RNA For Transcription: ● ● ● ● cytosine (C) - guanine (G) adenine (A)- uracil (U) Initiation is determined by the recognition of the promoter sequence in the DNA by the RNA polymerase. Stef The transcription start site is downstream of the promoter and is designated as the +1 site. Aspartic acid Alanina Valine Arginine Serine Lysine Asparagine Glutamic TEOPO|0C|AGUCAG|UC|AG/DCAG/3G/ CAGUC UGU A C A Threonine G Methionine Isoleucine…8:47 ll 5G% 4 Chapter 9 DNA worksheet 2022.d... 2. Write the mRNA transcript from the DNA template in question 1. Remember your enzyme for RNA polymerase must add RNA nucleotides starting with the 5' direction and moving to the 3' direction. So for the MRNA copy the strand of DNA that is 3'-5') 3. Refer to the codon chart at the bottom of the page. Translate the mRNA into a polypeptide using the following rules. (2 points) Direction of translation of mRNA is from 5' to 3'. 1. MRNA codons are read in groups of three nucleotides. 3. Translation must begin at the START codon. 4. the STOP codon terminates translation. 2. THIRD LETTER SECOND LETTER U TulUUU Phe UUC Phe UUA Leu UUG Leu A UGU Cys U JUGC Cys C UCU Ser UAU Tyr UCC Ser UAC Tyr UCA Ser UAA STOP UGA STOPA UCG Ser UAG STOPUGG Tp G CCUU Leu CÚC Leu CỦA Leu CUG Leu AJAUU Ile AUC Ile AUA Ile AUG Met STARTACG ThrAAG Lys GGUU Val GUC Val GUA Val GUG Val CCU Pro CAU His CCC Pro CAC His CCA Pro CAA Gln CCG Pro CAG Gln ACU ThrAAU Asn AGU…