A unique aquatic plant was discovered from a lagoon in El Nido, Palawan. To determine the protein content of the plant, an adequate amount of the plant extract was acquired, and Bradford assay was performed. Determine the total protein concentration, in µg/mL, of the acquired plant extract. A bovine serum albumin (BSA) stock solution with a concentration of 250 µg/mL was used and mixtures with the following compositions and absorbance readings were prepared:
A unique aquatic plant was discovered from a lagoon in El Nido, Palawan. To determine the protein content of the plant, an adequate amount of the plant extract was acquired, and Bradford assay was performed. Determine the total protein concentration, in µg/mL, of the acquired plant extract. A bovine serum albumin (BSA) stock solution with a concentration of 250 µg/mL was used and mixtures with the following compositions and absorbance readings were prepared:
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 7.2P
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Transcribed Image Text:A unique aquatic plant was discovered from a lagoon in El Nido,
Palawan. To determine the protein content of the plant, an
adequate amount of the plant extract was acquired, and Bradford
assay was performed. Determine the total protein concentration,
in µg/mL, of the acquired plant extract. A bovine serum albumin
(BSA) stock solution with a concentration of 250 µg/mL was used
and mixtures with the following compositions and absorbance
readings were prepared:
Volume of BSA
(mL)
Volume of water
(mL)
Absorbance
Tube #
at 595 nm
1
0.00
2.00
0.000
2
0.20
1.80
0.112
3
0.40
1.60
0.225
4
0.60
1.40
0.318
5
0.80
1.20
0.432
6
1.00
1.00
0.551
The absorbance reading of the plant extract is 0.275.
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