A certain medium has the following composition:Glucose 15 gYeast extract 5 gPeptone 5 gKH2PO4 2 gDistilled water 1,000 mla. Tell what chemical category this medium belongs to, and explainwhy this is true.b. How could you convert Staphylococcus medium intoa nonsynthetic medium?
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A certain medium has the following composition:
Glucose 15 g
Yeast extract 5 g
Peptone 5 g
KH2PO4 2 g
Distilled water 1,000 ml
a. Tell what chemical category this medium belongs to, and explain
why this is true.
b. How could you convert Staphylococcus medium into
a nonsynthetic medium?
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- A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table above) into a non-synthetic medium? a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? One that lives in the human stomach? Why are intestinal bacteria able to grow on media containing bile?If the volume of a Staphylococcus aureus cell is estimated at 0.5 μm3, how many cells could be accommodated, in principle, in 5 mL of saturated culture? (1 mL = 1 cm3). Show your calculations.Imagine you have been given a liquid culture of yeast with a starting concentration of 3.67 x 10' cells/ml and are asked to carry out the sample dilution process shown in the figure below. 100μl 100μl 100μl 100μl 100μl 0.9ml 0.9ml 0.9ml H2O H₂O 6.9ml 0.9ml H₂O H₂O H₂O Original 10-1 102 10-3 104 Culture 105 100μl 100μl 100μl Plate A Plate B Plate C a. How many colonies should have been present on Plate A in this example? - Answers must be whole numbers as partial colonies are not expected. b. Imagine you carried out the same dilution scheme shown in the figure above, but now, you do not know the concentration of the original culture. If you counted 163 colonies on Plate B, what is the concentration of cells/ml in the original culture?
- An autoclave is typically used for sterilization of media. a. Define sterilization. b. Under what conditions are media typically sterilized in an autoclave? c. What type of media components cannot be sterilized in an autoclave? d. What is an alternative to autoclaving for sterilizing heat-sensitive materials?Explain how to prepare 250 ml liquid and 500 ml solid form separately from LB (Luria Bertani) medium whose components and concentrations are given below. Yeast extract 5 g/L Peptone 10 g/L NaCl 10 g/L Agar 1.5 g/L. A certain medium has the following composition: Glucose 15 g Yeast extract 5 g Peptone 5 g KH2PO4 2 g Distilled water 1,000 ml Tell what chemical category this medium belongs to, and explain why this is true. How could you convert Staphylococcus medium (table 3.6A) into a nonsynthetic medium? a. Name four categories that blood agar fits. Alpha hemolysis Beta hemolysis Gamma Alpha prime Name four differential reactions that TSIA shows. Observe figure 3.15. Suggest what causes the difference in growth pattern between nonmotile and motile bacteria. Explain what a medium that is both selective and differential does, using figure 3.18. a. What kind of medium might you make to selectively grow a bacterium that lives in the ocean? MSA Mannitol salt agar One that lives in the human stomach? Why are intestinal bacteria able to grow…
- a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.Helicobacter pylori, a bacterium that causes stomach ulcers, can grow in medium with a pH above 5. It can survive but not grow in pH 2 medium as long as the medium contains Urea. You inoculate a pH 2 broth with 1,000,000 H. pylori and for 8 hours the organism did not grow. The next morning however, the medium has become quite turbid, suggesting that the organism has grown. Explain how this could happen. What phylum and class does this organism belong?Why would the following medium not be considereda chemically defined medium: glucose, 5 grams (g);NH4Cl, 1 g; KH2PO4, 1 g; MgSO4, 0.3 g; yeast extract, 5 g;distilled water, 1 liter? What is aseptic technique andwhy is it necessary?
- Why might clinical medicine have an interest in understanding bacterial cell division at the molecular level? Explain why a hyperthermophile would probably not be a human pathogen. Describe four factors that may have an influence on the effectiveness of an antimicrobial treatment. Explain why 70% or 80% alcohol is more effective than 100% alcohol in controlling microorganisms.Nitrate reduction test can be used to differentiate between Pseudomonads and Enterobacteriaceae. You inoculated your Nitrate broth with your unknown; after incubation, you added 5 drops of reagent A and 5 drops of reagent B. You observe a red color within 2 minutes. Is this a + or a - reaction? Assuming you did not get a red color, you then added a pinch of Zinc and this time tou obtained a red color. What does this mean?Can xylane powder from beechwood be dissolved in M9 media? I'm evaluating the production of hydrogen with E. coli (it has a construction that alows this strain to depolimerize xylane into xylose monomers by an endoxylanase), but i have a limited amount of this xylane, and i read TB, AM1 & M9 mediums were used, but they don't say if they just poured the xylan powder into the media solution, i also read they autoclaved it with a soft acid solution and then neutralized it with a basic soluction, but this is an acid hydrolyzate (AH) plus enzymatic hydrolysis (EH). It ´s certain that using both would enhance available xylose concentration, but i want to evaluate EH by itself, and i still can't find if it is just added as powder to the media. Would it dissolve? do i have to add some kind of buffer to the media?.