(a) (' . Hill plots. Label the axes and indicate on both the plot above and the Hill plot where the value of the dissociation equilibrium constant Ka for O2 binding is defined for 0 and 100 Torr of dichloromethane For the curves at 0 and 100 Torr of dichloromethane, draw their equivalent in the form of Ка Hb(O2)n nO2 +
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- 5. Anesthetic gases used in surgery are known to bind to the hemoglobin molecule in red blood cells. The diagram below illustrates O2 binding curves of normal human HbA in the presence of the anesthetic gas dichloromethane (DCM). O UNTREATED 100 a DCM 23 Tor • DCM 50 Torr x DCM 100 Torr Symbols: o, 0 Torr DCM; O, 23 Torr DCM A, 50 Torr DCM x, 100 Torr DCM. 80 40 (1 Torr = 1 mm Hg.) The solutions buffered to pH 7.4. 20 were 0.5 1.0 1.5 2.0 log po2 % OXYGENATIONCarbon monoxide binds the heme of hemoglobin in the same position as O2; however, it binnds 200 times better than 02 If we use a value of 40 torr for hemoglobin's Pso for 0z. what is its P5o for CO? 0.05 torr 0.20 torr 5 torr 200 torr O 8,000 torr5. Anesthetic gases used in surgery are known to bind to the hemoglobin molecule in red blood cells. The diagram below illustrates O2 binding curves of normal human HbA in the presence of the anesthetic gas dichloromethane (DCM). O UNTREATED 100 Symbols: a Dсм 23 Torr • DCM 50 Torr x DCM I00 Torr o, 0 Torr DCM; О, 23 Torr DСМ Д, 50 Torr DСМ x, 100 Torr DCM. 80 60 40 (1 Torr = 1 mm Hg.) 20 The solutions were buffered to pH 7.4. 0.5 1.0 1.5 2.0 log p02 For the curves at 0 and 100 Torr of dichloromethane, draw their equivalent in the form of (a) (* Hill plots. Label the axes and indicate on both the plot above and the Hill plot where the value of the dissociation equilibrium constant Kd for O2 binding is defined for 0 and 100 Torr of dichloromethane Kd Hb(O2)n nO2 Hb + Be sure to label the axes, indicate the units on both axes, and make the Hill plot so that the values of Kd are equivalent to those on the saturation plot above. Indicate whether cooperativity, as indicated by the Hill…
- Determine the concentration of [Ser] at 75s at T=335K time (s) [Ser] at T=335 0.150 5 0.143 20 0.120 40 0.090 90 0.015 0.005 0.047 M 0.038 М 0.088 МWhat are the differences in terms of structure and ligand-binding interactions? What are the functions of each type of GPCR?Q) Following an intensive course of chemotherapy, a 48-year-old woman had a thorough laboratory evaluation, selected results of which are: Hemoglobin 8 g/dL, hematocrit 32% Arterial blood gases: pH 7.38, Pa.CO₂ 42 mm Hg, bicarbonate 25 mEq/L, Pa.O2 62 mm Hg, O₂ saturation 92%. Given that the O2 binding capacity of hemoglobin is 1.34 ml/g, calculate the O2 capacity of her hemoglobin. A) 9.86 ml/dL B) 9.86 mm Hg C) 9.86 mEq/L D) 10.72 ml/dL E) 10.72 mEq/L Ans: D 6.11. Following an intensive course of chemotherapy, a 48-year-old woman had a thorough laboratory evaluation, selected results of which are: Hemoglobin 8 g/dL, hematocrit 32% Arterial blood gases: pH 7.38, Pa.CO₂ 42 mm Hg, bicarbonate 25 mEq/L, Pa.O₂ 64 mm Hg, O₂ saturation 92%. Given that the O2 binding capacity of hemoglobin is 1.34 ml/g, calculate the O2 content of her arterial hemoglobin. A) 9.86 ml/dL B) 9.86 mm Hg C) 9.86 mEq/L D) 10.72 ml/dL E) 10.72 mEq/L Ans: A
- Phenylephrine and nafazolin solutions are used as decongestants to relieve nasal congestion. Your patient has been given the following prescription below. You need to prepare 100 g of this nasal spray and make it isotonic. 1. There is an available 25% w/w Phenylephrine solution in the pharmacy. How many g of this solution will you need to supply the needed 0.5% of the patient? A. 1.0 g В. 1.5 g С. 2.0 g D. 4.0 g 2. Given the amount calculated on the previous item, and if the E value of phenylephrine HCl is 0.32, calculate its corresponding sodium chloride tonicic equivalent. А. 0.16 B. 0.64 С. 1.60 D. 6.40 3. If the E value of nafazolin is 0.27, what is the sodium chloride tonicic equivalent of the patient's needed nafazolin? A. 0.0135 B. 0.1350 1.3500 D. 13.500 4. What is the combined sodium chloride tonicic equivalent of the remaining ingredients (edetate disodium (E =0.24) and sodium metabisulfite (E = 0.7)? A. 1.060 B. 0.106 С. 1.290 D. 0.129 5. To make the solution isotonic, what…{1q}. 1. Why is cyanmethemoglobin method preferred than other methods? 2. In what circumstances is the copper sulfate method used? 3. Discuss the synthesis and breakdown of hemoglobin.?The figure shows three oxygen binding curves for hemoglobin in the red blood cells of normal blood, pH 7.6. The middle-dashed line is the standard line for human hemoglobin. Lines A and B represent two experimental conditions your lab partner plotted but forgot to label. 1 0.8 0.6 0.4 0.2 | pO2 Q. which one is the hemoglobin you reduced the concentration of 2,3- bisphosphoglycerate in? and which line is the data from the hemoglobin you changed the pH to 7.3?
- pO2 in tissues p02 in lungs 1.00 Oxygen-binding curves for normal Normal Hb hemoglobin, 50% carboxyhemoglobin, and hemoglobin from 0.8 an anemic individual. 0.6 Y 50% COHB 0.4- Anemic individual 0.2 4 8 12 pO2 (kPa) Box 5-1 Figure 2 Lehninger Principles of Biochemistry, Seventh Edition O 2017 W. H. Freeman and Company r aye i UIE I. Answer the following questions. Do not forget the units. 1) From the binding curve for normal Hb shown above, what is the Ka of normal hemoglobin?IV D5W/NS with 20 mEq KCL 1,000 mL/8 hr Allopurinol 200 mg PO tid Fortaz 1 g IV q6h Aztreonam (Azactam) 2 g IV q12h Flagyl 500 mg IV q8h Acetaminophen two tablets q4h prn A.Calculate mL/hr to set the IV pump. B. Calculate how many tablets of allopurinol will be given PO. Supply: 100 mg/tablet. C. Calculate how many mL/hr to set the IV pump to infuse Fortaz. Supply: 1-g vial to be diluted 10 mL of sterile water and further diluted in 50 mL NS to infuse over 30 minutes. D. Calculate how many mL of aztreonam to draw from the vial. Supply: 2-g vial to be diluted with 10 mL of sterile water and further diluted in 100 mL NS to Infuse over 60 minutes. E. Calculate how many mL/hr to set the IV pump to infuse Flagyl. Supply: 500 mg/100 mL to infuse over 1 hour.A patient presents to the emergency department in a critical condition. The patient’s blood was tested and the concentration of carbonic acid (H2CO3) was found to be 4.0×10-4 M and bicarbonate ion (HCO3-) 3.062×10-3 M. Ka = 4.3 × 10-7 H2CO3(aq) ⇌ HCO3-(aq) + H+(aq) Show all calculations including equation(s) used. Calculate the pH of the patients’ blood. Based on your answer would you say the patient is suffering from Acidosis or Alkalosis?