7. · In the space provided, sketch a titration curve (pH vs H+ ions dissociated) of a phosphate buffer shown below. The pKa of H2PO4¯ is 7.0. H,PO, HPO,2 + H*
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- Blood pH is maintained at a range of 7.4. The following set of equations representsthe reactions of the blood buffering system. The pk, for the dissociation ofH,COg into HOg and H* is 6.3.Carbonic AnhydraseCO2 + H2OH2CO3> HCO3- + H+(a) (Calculate the ratio [HCO3 ]/[H,COg] at physiological pH. Show work.(b)) An individual with diabetes presented at an urgent care with fatigue andnausea. Lab tests showed that their blood [HOg ]/[H,COg] ratio was 3.4. Whatwas the pH of the patient's blood? Show work.(c) ) Clinic staff also noticed that the patient was breathing rapidly. What isthe reason for this rapid breathing and how might it help alleviate the patient'scondition?At what pH does a lysine solution exhibit the highest buffering capacity? (Lysine pKas: pk1 = 2.2, pK2 = 8.95, pK3 = 10.5) %3D O рH 12.1 О рH 9.72 O pH 5.67 pH 8.951 Given a patient with a pCO2 of 35 mm Hg,and a pH=7.25 what is the bicarbonate cocentration? 2 In respiratory acidosis whar do each of the ciritical parameters in the Hendseron Haselbach Equation either rise or fall: pCO2 Write the equation first before pH trying to solve the problem, 3. Calculate the serum osmolality of a sample with Na=149 mmol/L Glucose=115mg/dl BUN=20 mg/dl 4.Calculate the anion gap of a sample with the following Na--147 CI-110 mmol/L HCO3--35 mmol/L/ 5. Calculate the LDL chloresterol of a samples with Cholesterol 255 mg/dl HDL Cholesterol 30 mg/dl and Triglyceride 250 mg/dl?_
- 10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944You have been provided with stock solutions of: stock A: 0.06 M sodium pyrophosphate buffer pH 8.5 stock B: 3 M ethanol stock C: 0.015 M NAD+ stock D: milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL Show your calculations to arrive at your answers.Polyprotic acids such as H3PO4, can act as acid-base buffers only in combination with polyprotic bases B if their concentration is kept low at pH values around neutrality at pH values around any of their pka's
- at 257 nm) is dissolved in 2.5 mL of buffer. A 250 microliter aliquot is removed and placed in a 1.0 cm A sample of adenosine triphosphate (ATP) (MW 507, ɛ = 14,700 M'cm cuvette with sufficient buffer to give a total volume of 2.0 mL. The absorbance of the sample at 257 nm is 2.0. Calculate the weight of ATP in the original 2.5 mL sample. (Choose the correct value from the drop-down list provided. The values of weight are listed in units of milligrams.) Weight of ATP: v mgCAR) All una Red stock soluton concentration: wis strue Somple #yelume dilution 1 ete total tock AR volume 50.00m 50. 00mL 50.00L AR COC conentrastion Absorbance Q dilutibn 2 dilution 3 10 O. 16.4 20 0. 237 turn dilution 4 25 So00 O. 392 shift unknarn 1 0.485 (Include a printed copy of the colibratlon curve) Colibrotion urve cayotion: R2 valve1- Using the pH 12 of the buffer and the pKa = 12.32. What is the molar ratio of A- to HA. 2- What is the molar amount of A- and HA needed to prepare a 0.2 M buffer? 3- How many grams of A- and HA are needed to prepare 40 mal of the buffer? The molar mass of HA is 142g/mol The molar mass for A- is 380.1g/mol
- Draw titration curve for compound X when NaOH equivalence(s) is/are added. Indicate the buffering region(s) with a box.80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.1) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula. 2)Calculate the required gram of EDTA to prepare O.5M, 100 ml EDTA (EDTA stock) mol and Molarity formula. 3.) Calculate the required volume of Tris Stock to prepare buffer A which contains 25 mM Tris-Hcl with final volume is 100 ml by using M1V1 =M2V2 formula 4) Calculate the required volume of EDTA stock to prepare buffer A which contains 10 mM EDTA with final volume is 100 ml by using M1V1 =M2V2 formula 5)Do the same for Buffer B calculations