6. Malate dehydrogenase catalyzes the following reversible reaction: NAD+ NADH + H* IZI malate dehydrogenase COO- HO-C-H CH₂ COO™ L-Malate COO- CH₂ Malate + NAD →NADH + H+ + oxaloacetate COO- Oxaloacetate AG' = 29.7 kJ/mol Calculate AG" and the ratio or products and reactants for the malate dehydrogenase reaction to proceed from left to right as shown. (The Faraday constant, , is 96.48 kJ/V-mol; RT(37°C) = 2.58kJ/mol) Steps: 1. Explain how you determined which molecule is an electron donor Malate and which is an acceptor NAD*. -2- 2. Calculate AED (write equation, then show calculations, for standard reduction potentials (E_values) see table in the posted lecture) 3. Calculate AG (write equation, then show calculations) 4. Calculate the ratio of products and reactants needed to for Malate + NAD+→NADH + H+ + oxaloacetate reaction to proceed forward (write equation, then show calculations)

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter20: Electron Transport And Oxidative Phosphorylation
Section: Chapter Questions
Problem 9P
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6. Malate dehydrogenase catalyzes the following reversible reaction:
COO-
HO-C-H
CH₂
COO™
L-Malate
NAD+
y
malate
NADH + H+
dehydrogenase
COO-
0=C
CH₂
COO™
Oxaloacetate
AG'° = 29.7 kJ/mol
Malate + NAD+→NADH + H+ + oxaloacetate
Calculate AG" and the ratio or products and reactants for the malate dehydrogenase reaction to proceed from
left to right as shown.
(The Faraday constant. 3, is 96.48 kJ/V-mol; RT(37°C)= 2.58kJ/mol)
Steps:
1. Explain how you determined which molecule is an electron donor Malate and which is an acceptor NAD*.
-2-
2. Calculate AED (write equation, then show calculations, for standard reduction potentials (E_values) see table
in the posted lecture)
3. Calculate AG (write equation, then show calculations)
4. Calculate the ratio of products and reactants needed to for Malate + NAD+→→NADH + H+ + oxaloacetate
reaction to proceed forward (write equation, then show calculations)
Transcribed Image Text:6. Malate dehydrogenase catalyzes the following reversible reaction: COO- HO-C-H CH₂ COO™ L-Malate NAD+ y malate NADH + H+ dehydrogenase COO- 0=C CH₂ COO™ Oxaloacetate AG'° = 29.7 kJ/mol Malate + NAD+→NADH + H+ + oxaloacetate Calculate AG" and the ratio or products and reactants for the malate dehydrogenase reaction to proceed from left to right as shown. (The Faraday constant. 3, is 96.48 kJ/V-mol; RT(37°C)= 2.58kJ/mol) Steps: 1. Explain how you determined which molecule is an electron donor Malate and which is an acceptor NAD*. -2- 2. Calculate AED (write equation, then show calculations, for standard reduction potentials (E_values) see table in the posted lecture) 3. Calculate AG (write equation, then show calculations) 4. Calculate the ratio of products and reactants needed to for Malate + NAD+→→NADH + H+ + oxaloacetate reaction to proceed forward (write equation, then show calculations)
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