In this FRET data table, an antibiotic was added, but the type was not recorded. According to the results, could the buffer being used be either negamycin, tetracycline, rifamycin, or cycloheximide? Explain why based on the FRET data.
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- The figure above depicts an agar cube with a side length of 13\, \text{mm}13mm13, start text, m, m, end text. In an experiment, students submerged the cube in red dye for 121212 hours. The red dye permeated 1\, \text{mm}1mm1, start text, m, m, end text on each side, as indicated by the shading in the figure. Volume of a rectangular solid: V = lwhV=lwhV, equals, l, w, h Calculate the volume of the agar cube that remained unpenetrated by the red dye.Plating 0.1 mL of a sample diluted by a factor of 10^(-3) produced 43 colonies. What was the original concentration (OCD) in the undiluted sample?A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.1mL Stock #1 1mL 2000060 9mL #2 9mL wwwww #3 4. 1mL 0000000 A 9mL wooooo #4 0.1m/L O 1mL 5000000 B 9mL #5 1mL 1mL 9mL wwwwww #6 0.1mL 1mk O. D Using the picture serial dilution scheme and the following information (plate A has 276 colonies, plate B has 298, plate C has 2, and plate D has 30), calculate the average number of colony forming units per mL in the stock tube. Make sure to only use countable plates. Round your answer to the nearest one. Write only the number with any needed commas or decimals. Do not include units.2.1The number of bacteria in the samples need to be quantified. The laboratory uses a specificprotocol where the dilution factor on the spread plates must be:10-6; 10-7; 10-8Describe how you would prepare the required dilution series using the least amount of dilutionwater. 2.2 Calculate the amount of bacteria in the sample from the following results (show the steps in the calculation): Dilution factor Count10-6 27110-7 3510-8 21
- G418 disulfate (FW 692.7) is an antibiotic used to select transfected mammalian cells in tissue culture. The standard working concentration for selection is 400 µg/mL of active ingredient in culture medium. The bottle states that it contains 71% active ingredient. You need to make a 100x stock in medium and adjust the pH before you apply it to cells. How much powder should be added to make 10 mL of stock solution? How much do you add to a 2 mL culture dish?Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp and 100micro/ml of Kan final concentration. A 100ml of LB is provided and amp of Kan stock at 100 mg/ml and 50 mg/ml are also provided. Find how much each antibiotic stock solution is needed to add 100ml of LB to reach desired antibiotic concentration.Normal text Calibri BI UA G |EE| +2 I +3+1 CICCO4, 1 LE | ZJ VITICHI SUpstances did not test positive for any of the organic compound Distilled water did not test positive for any of the organic compounds. 5| 6 3) 4) What do all of the indicators you have used have in common? Indicators are all substances whose solutions change color due to changes in p 5) Is there a macromolecule that the tests in this lab did not test for No, there is no macromolecule that the lab did not test for. 6) Which compound is most common in foods that come from plants? Cellocuse is the single most common biochemical compound in living things. 7) If you wanted to reduce the amount of fat in your diet, what foods would you avoid? Steer away from foods high in saturated and trans fats like fried foods, fatty meats, stick margarine, snacks and items with hydrogenated oil. M 6 8) Which foods tested would your body use for a quick burst of energy? a. For energy when no carbohydrates are available? b. For building…
- The original concentration in a sample of kombucha is 2.79 x 10^6 CFU/ml. Which dilution would yield a countable plate? How would you make this? Show your calculations. Confused as to what's implied by "which dilution"? does it mean, how many times?Streptomycin sulfate 400 mg IM daily is ordered for a child weighing 77lbs. The recommended dosage is 20-40 mg/kg/day once daily. A 1 gram vial of streptomycin sulfate is available in powdered form with the following instructions: Dilution with 1.8 mL of sterile water will yield 400mg per mL. If safe, how many mLs will you give?A sample to be used for medical imaging is labeled with 18F,which has a half-life of 110 min. What percentage of the originalactivity in the sample remains after 300 min?