Answered: 5. Coomassie Brilliant Blue G-250, a… | bartleby

Biomedical Instrumentation Systems

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 5Q

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567write true if the statement is correct, otherwise, change the underlined word/phrase to make it correct. do not use abbreviations please answer all

5. Coomassie Brilliant Blue G-250, a dye used in the Bradford assay, has a maximum absorbance at 595
nm under acidic pH.
6. The graph of solubility vs pH results in a vertical parabola with a maxima near the sample's isoelectric
pH.
7. Coomassie Brilliant Blue, a reagent or standard that is usually used to determine the void volume of
a gel filtration chromatography column, has an approximate molecular weight of 2,000,000 Da.

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Hi, I just need some help calculating the final concentration for each of the standard solutions I made. The concentration of my stock solution is 0.1mM.
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Large proteins travel/migrate faster in both gel filtration chromatography and SDS- PAGE. O True O False

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