4. Using a fluorescent model substrate, you study the kinetics of an enzyme-catalyzed reaction. You observe the below data. Write an equation describing reaction velocity (v) versus [S]. Define and provide a numerical value for any constants that you include in your equation. v/ μM min-¹ 16 14 12 10 8 6 4 2 0 + 0 0.0 0.5 1.0 1.5 [S]/mM 2.0 In pilot experiments, you add the putative inhibitor imidazole reaction velocities: Doesn't affect anything 2.5 -N 0 3.0 3.5 H at 50 mM and observe the following [S] = 1 mM5 μM min-1 [S] = 2 mM9 μM min-¹ [S] =4 mM 11 μM min-1 Is imidazole a competitive or non-competitive inhibitor? Provide a brief explanation. < 1 1 6 >
4. Using a fluorescent model substrate, you study the kinetics of an enzyme-catalyzed reaction. You observe the below data. Write an equation describing reaction velocity (v) versus [S]. Define and provide a numerical value for any constants that you include in your equation. v/ μM min-¹ 16 14 12 10 8 6 4 2 0 + 0 0.0 0.5 1.0 1.5 [S]/mM 2.0 In pilot experiments, you add the putative inhibitor imidazole reaction velocities: Doesn't affect anything 2.5 -N 0 3.0 3.5 H at 50 mM and observe the following [S] = 1 mM5 μM min-1 [S] = 2 mM9 μM min-¹ [S] =4 mM 11 μM min-1 Is imidazole a competitive or non-competitive inhibitor? Provide a brief explanation. < 1 1 6 >
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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