4. Using a fluorescent model substrate, you study the kinetics of an enzyme-catalyzed reaction. You observe the below data. Write an equation describing reaction velocity (v) versus [S]. Define and provide a numerical value for any constants that you include in your equation. v/ μM min-¹ 16 14 12 10 8 6 4 2 0 + 0 0.0 0.5 1.0 1.5 [S]/mM 2.0 In pilot experiments, you add the putative inhibitor imidazole reaction velocities: Doesn't affect anything 2.5 -N 0 3.0 3.5 H at 50 mM and observe the following [S] = 1 mM5 μM min-1 [S] = 2 mM9 μM min-¹ [S] =4 mM 11 μM min-1 Is imidazole a competitive or non-competitive inhibitor? Provide a brief explanation. < 1 1 6 >

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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4. Using a fluorescent model substrate, you study the kinetics of an enzyme-catalyzed reaction. You observe the
below data. Write an equation describing reaction velocity (v) versus [S]. Define and provide a numerical value for
any constants that you include in your equation.
16
14
12
4
2
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
[S] / mM
`N'
at 50 mM and observe the following
In pilot experiments, you add the putative inhibitor imidazole
Doesn't affect anything
1
reaction velocities:
[S] = 1 mM5 µM min-1
[S] = 2 mM9 9 µM min-1
[S] = 4 mM 911 µM min-1
Is imidazole a competitive or non-competitive inhibitor? Provide a brief explanation.
>
9 00
v/ µM min-
Transcribed Image Text:4. Using a fluorescent model substrate, you study the kinetics of an enzyme-catalyzed reaction. You observe the below data. Write an equation describing reaction velocity (v) versus [S]. Define and provide a numerical value for any constants that you include in your equation. 16 14 12 4 2 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 [S] / mM `N' at 50 mM and observe the following In pilot experiments, you add the putative inhibitor imidazole Doesn't affect anything 1 reaction velocities: [S] = 1 mM5 µM min-1 [S] = 2 mM9 9 µM min-1 [S] = 4 mM 911 µM min-1 Is imidazole a competitive or non-competitive inhibitor? Provide a brief explanation. > 9 00 v/ µM min-
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