Answered: + 3. The following results were… | bartleby

Curren'S Math For Meds: Dosages & Sol

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 3SST

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b. Which chemotherapeutic drug above (A, B, C, or D) is most effective against Staphylococcus aureus, if possible? Explain your answer.

+
3. The following results were obtained from a disk diffusion test for a strain of Escherichia coli.
Chemotherapeutic
Zone of inhibition (mm)
A
B
7
10
C
D
18
3
Which drug above (A, B, C, or D) is most effective against this E. coli strain?
Which drug above (A, B, C, or D) is most effective against Staphylococcus aureus? Explain your
answer.

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1. The antibacterial activity of flavonoids was tested against the bacterial strain, Escherichia coli. The minimum inhibitory concentration at which 50% of the compounds were inhibited (MIC50) are presented in the table below for some of the compounds. Table 1: Antibacterial activity of flavonoids tested against E. coli Compound Kaempferol Quercetin Compound X Luteolin Tangeritin 5,6,7,4'- Tetramethoxyflavone Genistin Genistein MIC50 (µg/mL) 25.50 35.76 36.72 67.25 137.10 156.34 b) 5,6,7,4'-Tetramethoxyflavone and tangeritin. c) Genistein and genistin. d) Genistein and kaempferol. 238.94 320.88 1.2 Using structure-activity relationships, explain the differences in antibacterial activity between the following compounds: a) Quercetin and luteolin.
Nitrate reduction test can be used to differentiate between Pseudomonads and Enterobacteriaceae. You inoculated your Nitrate broth with your unknown; after incubation, you added 5 drops of reagent A and 5 drops of reagent B. You observe a red color within 2 minutes. Is this a + or a - reaction? Assuming you did not get a red color, you then added a pinch of Zinc and this time tou obtained a red color. What does this mean?
A researcher is interested to test the antibacterial activity of a Philippine Plant crude extracts against Beta-lactamase producing Klebsiella pneumonia isolated in a hospital setting. He will use the following concentrations of crude extract, 500mg/ml, 250mg/ml and 100mg/ml. Positive control: Tetracycline 10mg/ml and DMSO as the negative control. Given this research scenario kindly construct a research framework.
Briefly discuss 2 fluorogenic methods that can be used in detecting coliforms and E.coli. Include the principle and positive results
1). SIM Tubes and TSI slants turn black if the organism is able to reduce iron compounds. For which organism(s) below would you expect a black reaction? Circle all that apply. Escherichia coli. Proteus vulgaris. Salmonella typhimurium. Alcaligenes faecalis. Shigella flexneri. 2). If Citrate is a sugar and fermentation generates an acid, why does the BromThymol Blue indicator in the media turn blue and not yellow? 3). Why is Salmonellosis associated with eggs? Can you get Salmonellosis from other animal sources?
Which of the following are positive for the production of indoor from the amino acid tryptophan?
Which of the following are positive for the production of indole from the amino acid tryptophan?
Given this research scenarios kindly construct a research framework. A researcher is interested to test the antibacterial activity of a Philippine Plant crude extracts against Beta-lactamase producing Klebsiella pneumonia isolated in a hospital setting. He will use the following concentrations of crude extract, 500mg/ml, 250mg/ml and 100mg/ml. Positive control: Tetracycline 10mg/ml and DMSO as the negative control.
Your colleague handed you a novel strain of coli that is purifying a protein with a 6xHisTag; they claim it is superior to the TOP10 cells you have been using. But even with a larger culture size, you discover that your protein yield—using the same Ni-NTA column—is quite low. You discover that the novel strain of Escherichia coli generates an unusually high quantity of dicarboxylic acids, a byproduct of the citric acid cycle that is recognized for its ability to function as an all-purpose metal chelator. What do you think the issue is with purifying IMAC protein with this new strain of E. coli?
One of the early results shows that the post-centrifugation pellet of encapsulated cells also contains EA1 and/or Sap. Why is this not proof that Bacillus anthracis cells have both an S-layer and a capsule simultaneously? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
One of the early results shows that the post-centrifugation pellet of encapsulated cells also contains EA1 and/or Sap. Why is this not proof that Bacillus anthracis cells have both an S-layer and a capsule simultaneously? I need help finding the answer in the article and explain in short answer link to article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106848/
E. coli has a doubling time of 0.345 hours at 37 0C. Starting with 1 milligram of cells, assuming a lag phase of 30 minutes and a stationary phase of 30 minutes, what number of cells would you expect at the end of 13h, assuming that besides the lag and stationary phases the culture is continuously in exponential phase. Assume that ln (2) = 0.69 and that the mass of a single cell is 10^(-12) g.

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