Q: a) Analyze the importance of dropping mercury electrode on polarography. (b) (i) Calculate the…
A: a) Importance of dropping mercury electrode on polarography : Dropping mercury electrode is a…
Q: Chromatography
A:
Q: In reversed phase HPLC, how will the retention time of a component of a mixture vary with the…
A: Retention time is a measure of the time that is taken by a solute to pass through a column.In…
Q: In TLC, why does the developing solvent 3:1:1 n-butanol/acetic acid/water climb up the TLC plate so…
A: Thin-layer chromatography (TLC)-It is a technique used for the separation of a mixture of volatile…
Q: Capillary electrophoresis differs from other chromatographic methods because:
A: It uses unique detectors.
Q: 10. Consider the following compounds when answering the questions below. Assume these compounds can…
A:
Q: It is not of the solute property detector used in HPLC is: a. Refractive index detector b. UV…
A: Mixture components are estimated using HPLC detectors. The actual separation in HPLC (High…
Q: What is chiral chromatography?
A:
Q: Which technique measures secondary structure? a. Size exclusion chromatography b. Circular Dichroism…
A: The secondary structure is formed by bending of the 1o structure. In this, an intermolecular force…
Q: Which one of the following approaches would be inappropriate for the introduction of the sample in…
A: ICP-MS is a type of mass spectroscopy which is used to ionize a sample.
Q: Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a…
A:
Q: .Which of the following solvents is not commonly used in reversed-phase HPLC? a) Ethanol Water b)…
A: The solvent which is not used commonly in HPLC has to be given,
Q: What are the principles involved in the separation of substances in Thin Layer Chromatography? 2.…
A: The question is based on the concept of organic reactions. We have to explain the principle of thin…
Q: You first separated the components of spinach and then identified them by TLC analysis. Was there a…
A: TLC of spinach means separation of chlorophyll pigment.The different form of chlorophyll found in…
Q: How can you use ion-exchange chromatography to de-ionize water?
A: How can you use ion-exchange chromatography to de-ionize water?
Q: Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse…
A: Reversed phase chromatography have long chain alkyl chains which are non polar in nature .
Q: Which of the following is not a method for measuring reaction rates? A) total pressure B) thin layer…
A: A multiple choice question based on measuring reaction rate, which is to be accomplished.
Q: PART1: Put True (T) or False (F) for the following sentences: 1. chromatography" derived from two…
A: 1. True 2. True 3. False 4. True 5.False
Q: Explain the term gradient elution mode in HPLC Differentiate between gel filtration and gel…
A: The term elution mode in HPLC is defined as the separation method where the compounds are located…
Q: Explain the term gradient elution mode in HPLC
A: Given : We have to tell about gradient elution term .
Q: 1. Define retention factor. Explain why it is or isn't an intrinsic property of a substance. 2. A…
A: TLC (Thin layer chromatography) is a technique used to separate the components in a mixture using a…
Q: 19. What is thin layer chromatography (TLC) LEAST useful for from the list below? A) To determine if…
A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Which mechanisms of band broadening that operate in chromatography are absent in capillary…
A: The process of band broadening is basically explained by the Van Deemter equation in chromatography…
Q: 1- What are the main two types of columns in the HPLC? 2-What are the most disadvantages of…
A: “Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: Briefly explain how cation exchange chromatography can used in protein separation?
A: Cation exchange chromatography is a form of ion exchange chromatography (IEX), which is basically…
Q: Which one of the following chromatographic techniques is useful in the identification of organic…
A: Chromatography is used to know the molecular weight . Here the known polymer is used to made the…
Q: 24. In paper chromatography, a rectangular piece of filter paper serves as the 24. A. mobile phase…
A: Paper chromatography was discovered by Synge and Martin in the year 1943. In paper chromatography,…
Q: In HPLC, why is purging or initialization being done before analysis of samples, explain.
A: ✓basically following two reasons for using purging and initialization during HPLC sample preparation…
Q: 4) If the classification of the chromatography run is a reverse-phase chromatography, discuss the…
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Q: Answer in your own words. What is the advantage of very small particle sizes in Liquid solid…
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Q: From the experiment in Question 1, the following data were obtained: 2. Distance traveled by spot O…
A:
Q: For what type of separation would you use ion exchange chromatography (IC)?
A: Ion exchange chromatography separates ions and polar molecules based on their affinity to the ion…
Q: Z and E -oct-3-en-2-ol is hard or easy to separate in thin layers in chromatography?
A: Greater the polarity difference between the two components of a mixture easier will be the…
Q: 4. a. What physical property of a compound predicts whether that compound travels slowly or rapidly…
A: Gas chromatography is an analytical method to separate the components of a mixture in a sample by…
Q: 4. Enumerate the differences between a serological and a volumetric pipette. 5. Enumerate the uses,…
A: Pipettes are one of the most important instruments in the analytical measurements that help us in…
Q: You were analyzing a mixture of volatile solvents using Gas Chromatography and found out that two…
A: Chromatography is the method for the separation of a mixture with very high resolution, which is an…
Q: . Compare HPLC, HPTLC and GC chromatography
A: Chromatography is a physical separation method that is used to separate and identify the components…
Q: 24. Define a) chiral chromatography. b) adsorption chromatography.
A: As per rule we have answer only one question , so I am answering 24 question
Q: Please propose a chromatography-based strategy to purify human insulin proteins that have been…
A:
Q: How does capillary zone electrophoresis (CZE) works.
A: Capillary zone electrophoresis is a technique used for separating the individual components from a…
Q: 35. The stationary phase, as well as mobile phase, are liquid in Adsorption column chromatography…
A: Answer: Chromatography is a separation technique, in which we separate the components of a mixture…
Q: What is the purpose of thin layer chromography lab? What is a hypothesis I can use when conducting a…
A: Introduction: Chromatography is a technique used to separate the mixture if solution. There are two…
Q: ) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions.…
A: The main components of clove oil are eugenol, eugenol acetate and caryophyllene. The structures of…
Q: What kind of chromatography would you for purification of full length antibody ?
A: Chromatography is used to seperate and purify mixture of species . Various type of chromatrographic…
Q: Why is thin layer chromatography a convenient way to anayize triphenylmethanol
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Q: Name types of gels (sieving matrix) that may be used for gel filtration chromatography and discuss…
A: The types of gels for gel filtration chromatography include, Dextran Polyacrylamide beads/ agarose…
Q: 1. In the chromatography plate there are three solutes that move forward: 2cm, 2.5cm and 3cm Which…
A:
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- In molecular exclusion (size exclusion) chromatography, the molecules have the longest retention times. a) the larger molecules b) the smaller moleculesHow can you use ion exchange Chromatography to de-ionize water? Please shortly write at your own words. (4-5 lines maximum ).1) Which technique would be best to determine the amount of nitrate in drinking water? Select one. a) Electrophoresis b) UV Spectroscopy c) Reverse osmosis d) Ion Chromatography e) Atomic Absorption 2) Which technique would be best to quantitatively determine the concentration of a substance that absorbs light in the visible range? a) HPLC b) Spectroscopy c) PCR d) Ion chromatography e) Ultracentrifugation
- What are the differences between Ultra-Performance Liquid Chromatography (UPLC) over High-performance liquid chromatography?As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True FalseEnumerate the differences between a serological and a volumetric pipette and enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.
- Qno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatographyDifferentiate between gel filtration and gel permeation chromatography.In normal-phase HPLC chromatography, the stationary phase is polar and the mobile phase is nonpolar. In the reversed phase we have just the opposite; the stationary phase is nonpolar and the mobile phase is polar. Describe molecules in which move faster or slower in each case.