2. What is the cfu/g or cfu/mL in each sample if the following counts were obtained after spread plating on Potato Dextrose Agar with chloramphenicol? Mongo bread 221, 257, 292 Melon juice Dilution TNTC, TNTC, TNTC 10-1 59, 41, 30 125, 132, 124 10-2 No growth 19, 17, 15 10-3
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- 5. Provided with the following data, compute the corresponding CFU/ml of the original culture. Assume that spread plate technique was done. Show your solutions. 10-1 10-2 10-3 10-4 10-5 Plate 1 TNTC 340 132 45 19 Plate 2 TNTC TNTC 242 80 25 Plate 3 TNTC 280 90 22 25. You were asked to prepare 250 ml of Acidified Potato Dextrose agar for the subculture of Alicyclobacillus sp. Given the composition of the medium below, compute for each component of Acidified PDA. :Components Amount per liter Potato Infusion 200.00 ml Dextrose 2.00% (w/v) Tartaric acid 1.00 ml of 10.00% solution (available as stock solution of 50,000 ppm) Agar 1.70% ‒‒‒‒‒‒‒‒‒‒‒‒‒ 5.3. Tartaric acid. Express your answers using two decimal places. Follow this format in typing your answers: answerabbreviated unit (e.g. 3.00 g. 0.75 ml, 10.25 mg) (2.5 pts).1. Name of the product 2. Predominant ingredient by weight 3. Serving size 4. How many total number of servings are there in one container 5. What is the fortification or enrichment status of the product 6. What is in one serving in terms of following criteria that is a) Is it high in particular nutrients if so which ones b) reliable source of any nutrients? If so, which ones? c) Reduced in calories or any nutrients? If so which ones? 7. Is your chosen product free of fat, sodium or sugar or other food nutrients If so, describe below: FREE OF LOW IN VERY LOW IN Attach the picture of label to the worksheet and upload Nutrition Class
- The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolismof 1 stion s page 30- F2 Which of the following terms is associated with the probability of a preparation not being sterile after it has been exposed to a sterilization process? Select one: Oa. Particulate Matter level Qb. Sterility Assurance level О с. Pyrogen Load level O d. Optimal Sterilization level F3 F4 F5 Ol پیار Q Search F6 F7 F8 F9 === 8 9 F10 Time left 22:34:03 F11 Next pageWhich of the following is the a-anomer? H но H Но H2C H ОН H2C- нн ОН со 4 1 2 1 Submit 3 -OH Н ОН -OH ОН Н there is no a-anomer ОН ОН Н Request Answer H Но НО Н H2C нн ОН 2 H2C ОН -OH 4 Н ОН н н H OH -О. ОН ОН Н ОН Н
- AY AY * 00 HI T 00 2 3. ment10: Connect Regis.. M https://Ims.mh.. nemical Content of Media i Saved Help Save & Exit Chec Chemically defined medium Complex medium 2 Enriched medium Fastidious Heterotroph 9 Autotroph Match each of the options above to the items below. Uses inorganic carbon as a carbon source for building its own organic molecules. A type of organism that has complex nutrient requirements and requires a nutritionally dense environment. A media containing extracts from other other organisms. A medium with specifically added nutrients to enhance the growth of fastidious organisms. An organism that uses organic carbon as its carbon source. A medium of which the exact chemical composition is known. MacBook Air DD F7 000 000 F2 F4 F5 F8 %23 2$ 4. 9- } 1 Y | 7.1. How many grams of dextrose will be needed in the final product? 2. How many mL of Dextrose 70% will be needed to make the final product? 3. How many mL of sterile water will be needed to make the final product?1. The prescription for an oral solution reads “1 tbsp ac & hs x 10d.” What is the minimum (in mL) of solution should you dispense? 2. A nursing floor requires half liter of 50% isopropyl alcohol. How many mL of 70% isopropyl alcohol will be needed for compounding this order? 3. How may grams of pure dextrose is needed to prepare 100cc of D50W?
- You are asked to make 10 mL of buffer from a 5X concentrate. invitrogen KP 0reen CR Te reorder visit themotshor.com For suppot visit mhe.com/suppot How many mL of concentrate do you require before diluting to a final volume of 10 mL with DDW?Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.A doctor had ordered 1 floz 4% medication syrup bid X 5d. How many floz are required in total? How many floz of each of two syrups that are 2% medication and 7% should be mixed to produce the 4% medication syrup?