2. Given the results table below, create a flow chart to differentiate the organisms so you could identify an unknown isolate out of these four possibilities. Organism Urease MR TSI Glucose fermentation JM02 + A/H₂S+ A SL65 + A/A Ag DT22 K/K K YS98 + - A/A Ag
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- 4. All the following can be autoclaved EXCEPT: a) Biological material b) Glass ware c) Radioactive material d) Broth and gel media for growing bacteria5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above22) Which of the techniques/characteristics below may be used to identify a target bacterium in a pool of microorganism? a) its morphology b) its growth requirement c) antibody-antigen interaction ) d) all of the above. ) e) a and c 23) What is the process that produces alcohol in S. cerevisiae (yeast)? () a) Respiration ( b) Sedimentation c) Photosynthesis )d) Fermentation 24) Identify the most mistaken (wrong) choice: a) Halophiles are those organisms that like high salt concentrations. ) b) Some bacteria reproduce by binary fission. ( c) A fungus cell contains a true nucleus. d) During the lag phase of growth, the metabolic activity is very low1. Give at least five observable metabolic activities or phenotypes of an organisms that are determined by the MicroSEQ™ ID microbial identification system. Please, do not just copy from somewhere. Answe this comprehensively.
- 2. A mannitol-salt agar plate was inoculated with these bacteria and is shown below. A. What type of organisms grow on this medium? B. Based on the reaction below, what can you say about the organism derived from the patient's abscess?22) Which of the techniques/characteristics below may be used to identify a target bacterium in a pool of microorganism? ( a) its morphology () b) its growth requirement c) antibody-antigen interaction d) all of the above. ( e) a and c 23) What is the process that produces alcohol in S. cerevisiae (yeast)? ( a Respiration ( b) Sedimentation c) Photosynthesis ) d) Fermentation1 (a)Why is the spectrophotometer set at 0.000 absorbance for the uninoculated tube of broth? (B) Why can't you use the same plot (or standard curve) of plot of absorbance versus cell count for Escherichia coli for other bacteria? (C)List 3 advantages of estimating microbial numbers or biomass by the turbidimetric method? (C)(ii)List 2 disadvantages of this method? (D) Can you measure all kinds of microbes this way? Why not? ( this is not a graded assignment)
- 1. Define the following terms: a. Chermolithoheterotroph b. Microaerophile c. Chermoorganoheterotroph d. Obligate anaerobe e. Facultative anaerobic f. Coliform5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts 6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above 7. The temperature to incubate the bacteria in the above mentioned experiment should be __: a) 22 oC b) 37 oC c) 98 oC d) 37 o1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…
- 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.1. How can zinc dust differentiate nitrate reductase positive- and nitrate reductase negative organisms? Answer this comprehensively.Why are the Methyl Red/Voges-Proskauer tests done after 48 hours of incubation?a) Readings taken at earlier time points may be different from readings taken at 48 hours b) Bacteria need 48 hours to process all the oxygen in the media c) Bacteria grow slowly in the MR/VP media d) K2HPO4 needs 48 hours to react with the acids produced by fermenting bacteria